ArticleMolecular metabolism2019
Comprehensive and quantitative analysis of white and brown adipose tissue by shotgun lipidomics.
Article in Molecular metabolism, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
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- A conserved ER protein prevents lipotoxicity by stimulating the key enzyme in glycerolipid synthesis.Nature communications · 2026Article
- An adipocyte-endothelial framework to identify molecular signatures of metabolic vulnerability in obesity.Research square · 2026Article
- Subcutaneous and visceral adipose tissue lipidome in children reveals novel lipid species involved in obesity.Journal of physiology and biochemistry · 2026Article
- Single-cell Raman imaging reveals fructose impairs brown adipocyte differentiation.Biosensors & bioelectronics · 2025Article
- Hypoglycemic mechanism of modified Gegen Qinlian decoction based on regulating the expression and DNA methylation of cholesterol transporters in the adipose tissue of type 2 diabetes mellitus rats.Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2025Article
- Dietary control of peripheral adipose storage capacity through membrane lipid remodelling.Nature metabolism · 2025Article
- Chemical Landscape of Adipocytes Derived from 3T3-L1 Cells Investigated by Fourier Transform Infrared and Raman Spectroscopies.International journal of molecular sciences · 2024Article
- Mitochondrial Dysfunction Associated with mtDNA in Metabolic Syndrome and Obesity.International journal of molecular sciences · 2023Review
- Anti-Obesity Effect and Mechanism of Chitooligosaccharides Were Revealed Based on Lipidomics in Diet-Induced Obese Mice.Molecules (Basel, Switzerland) · 2023Article
- Fatty acid desaturase 2 determines the lipidomic landscape and steroidogenic function of the adrenal gland.Science advances · 2023Article
- Transcriptomic and lipidomic profiling of subcutaneous and visceral adipose tissues in 15 vertebrates.Scientific data · 2023Article
- Remodeling of Adipose Tissues by Fatty Acids: Mechanistic Update on Browning and Thermogenesis by n-3 Polyunsaturated Fatty Acids.Pharmaceutical research · 2023Review
- Integrated Metabolomics, Lipidomics, and Genomics Reveal the Presence of a New Biomarker, Butanediol Glucuronide, Associated with the Activation of Liver Ketogenesis and Lipid Oxidation by Tomato-BasedAntioxidants (Basel, Switzerland) · 2022Article
- Mass Spectrometry-Based Lipidomics of Brown Adipose Tissue and Plasma of New-Born Lambs Subjected to Short-Term Cold Exposure.Animals : an open access journal from MDPI · 2022Article
- Article
- The foundations and development of lipidomics.Journal of lipid research · 2022Review
- Recombinant human GLP-1 beinaglutide regulates lipid metabolism of adipose tissues in diet-induced obese mice.iScience · 2021Article
- Opti-nQL: An Optimized, Versatile and Sensitive Nano-LC Method for MS-Based Lipidomics Analysis.Metabolites · 2021Article
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Authors and funding
8 authors.
Funding
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Abstract
objectiveShotgun lipidomics enables an extensive analysis of lipids from tissues and fluids. Each specimen requires appropriate extraction and processing procedures to ensure good coverage and reproducible quantification of the lipidome. Adipose tissue (AT) has become a research focus with regard to its involvement in obesity-related pathologies. However, the quantification of the AT lipidome is particularly challenging due to the predominance of triacylglycerides, which elicit high ion suppression of the remaining lipid classes.
methodsWe present a new and validated method for shotgun lipidomics of AT, which tailors the lipid extraction procedure to the target specimen and features high reproducibility with a linear dynamic range of at least 4 orders of magnitude for all lipid classes.
resultsUtilizing this method, we observed tissue-specific and diet-related differences in three AT types (brown, gonadal, inguinal subcutaneous) from lean and obese mice. Brown AT exhibited a distinct lipidomic profile with the greatest lipid class diversity and responded to high-fat diet by altering its lipid composition, which shifted towards that of white AT. Moreover, diet-induced obesity promoted an overall remodeling of the lipidome, where all three AT types featured a significant increase in longer and more unsaturated triacylglyceride and phospholipid species.
conclusionsThe here presented method facilitates reproducible systematic lipidomic profiling of AT and could be integrated with further -omics approaches used in (pre-) clinical research, in order to advance the understanding of the molecular metabolic dynamics involved in the pathogenesis of obesity-associated disorders.
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