ArticleBMC molecular biology2019
RNA sequencing, selection of reference genes and demonstration of feeding RNAi in Thrips tabaci (Lind.) (Thysanoptera: Thripidae).
Article in BMC molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Enhanced dsRNA Production via a Three-Terminator Vector and Transcriptomic Correlates of RNAi Exposure in Thrips.Insects · 2026Article
- Comparative transcriptome analysis of developmental stages and characterization of core RNAi-related genes in the spittlebug Mahanarva fimbriolata.BMC genomics · 2026Article
- Microinjection-enabled gene silencing in first instar larvae of western flower thrips, Frankliniella occidentalis, reveals vital genes for larval survival.Insect science · 2025Article
- Review
- Review
- Article
- Stable internal reference genes for quantitative RT-PCR analyses in Rhipicephalus microplus during embryogenesis.Ticks and tick-borne diseases · 2023Article
- Chloroplast Genome Engineering: A Plausible Approach to Combat Chili Thrips and Other Agronomic Insect Pests of Crops.Plants (Basel, Switzerland) · 2023Review
- Nanoparticle-Shielded dsRNA Delivery for Enhancing RNAi Efficiency in Cotton Spotted BollwormInternational journal of molecular sciences · 2023Article
- Effect of silencingFrontiers in microbiology · 2023Article
- Silencing of Thrips palmi UHRF1BP1 and PFAS Using Antisense Oligos Induces Mortality and Reduces Tospovirus Titer in Its VectorPathogens (Basel, Switzerland) · 2022Article
- RNA meets toxicology: efficacy indicators from the experimental design of RNAi studies for insect pest management.Pest management science · 2022Review
- Efficient control of western flower thrips by plastid-mediated RNA interference.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Reference Genes for Expression Analyses by qRT-PCR inInsects · 2022Article
- Innate and adaptive resistance to RNAi: a major challenge and hurdle to the development of double stranded RNA-based pesticides.3 Biotech · 2021Review
- Review
- Transcriptome analysis unravels RNAi pathways genes and putative expansion of CYP450 gene family in cotton leafhopper Amrasca biguttula (Ishida).Molecular biology reports · 2021Article
- Identification and Validation of Reference Genes for Gene Expression Analysis in Different Development Stages ofFrontiers in microbiology · 2021Article
- Next Generation dsRNA-Based Insect Control: Success So Far and Challenges.Frontiers in plant science · 2021Review
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThrips tabaci is a severe pest of onion and cotton. Due to lack of information on its genome or transcriptome, not much is known about this insect at the molecular level. To initiate molecular studies in this insect, RNA was sequenced; de novo transcriptome assembly and analysis were performed. The RNAseq data was used to identify reference and RNAi pathway genes in this insect. Additionally, feeding RNAi was demonstrated in T. tabaci for the first time.
resultsFrom the assembled transcriptome, 27,836 coding sequence (CDS) with an average size of 1236 bp per CDS were identified. About 85.4% of CDS identified showed positive Blast hits. The homologs of most of the core RNAi machinery genes were identified in this transcriptome. To select reference genes for reverse-transcriptase real-time quantitative PCR (RT-qPCR) experiments, 14 housekeeping genes were identified in the transcriptome and their expression was analyzed by (RT-qPCR). UbiCE in adult, 28s in nymphs and SOD under starvation stress were identified as the most stable reference genes for RT-qPCR. Feeding dsSNF7 and dsAQP caused 16.4- and 14.47-fold reduction in SNF7 and AQP mRNA levels respectively, when compared to their levels in dsGFP fed control insects. Feeding dsSNF7 or dsAQP also caused 62 and 72% mortality in T. tabaci. Interestingly, simultaneous feeding of dsRNAs targeting SNF7 or AQP and one of the RNAi pathway genes (Dicer-2/Aubergine/Staufen) resulted in a significant reduction in RNAi of target genes. These data suggest the existence of robust RNAi machinery in T. tabaci.
conclusionThe current research is the first report of the assembled, analyzed and annotated RNAseq resource for T. tabaci, which may be used for future molecular studies in this insect. Reference genes validated across stages and starvation stress provides first-hand information on stable genes in T. tabaci. The information on RNAi machinery genes and significant knockdown of the target gene through dsRNA feeding in synthetic diet confirms the presence of efficient RNAi in this insect. These data provide a solid foundation for further research on developing RNAi as a method to manage this pest.
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