ArticleACS omega2019
Benchmarking Smartphone Fluorescence-Based Microscopy with DNA Origami Nanobeads: Reducing the Gap toward Single-Molecule Sensitivity.
Article in ACS omega, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 63 citations in OpenAlex.
- Toward point-of-care cancer diagnostics: gold nanoparticle-based colorimetric sensors.RSC advances · 2026Review
- Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope.Nature communications · 2025Article
- Review
- Enhancing Biomarker Detection and Imaging Performance of Smartphone Fluorescence Microscopy Devices.Biosensors · 2025Article
- Accelerated Development of a COVID-19 Lateral Flow Test in an Academic Setting: Lessons Learned.Accounts of chemical research · 2024Article
- A commentary on the development and use of smartphone imaging devices.Biophysical reviews · 2024Article
- Article
- Fluorescence-Based Portable Assays for Detection of Biological and Chemical Analytes.Sensors (Basel, Switzerland) · 2023Review
- CRISPR-Enhanced Hydrogel Microparticles for Multiplexed Detection of Nucleic Acids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Rolling Circle Amplification Tailored for Plasmonic Biosensors: From Ensemble to Single-Molecule Detection.ACS applied materials & interfaces · 2022Article
- DNA origami book biosensor for multiplex detection of cancer-associated nucleic acids.Nanoscale · 2022Article
- Optical bio-sensing of DNA methylation analysis: an overview of recent progress and future prospects.RSC advances · 2022Review
- Recent Advances in Single-Molecule Sensors Based on STM Break Junction Measurements.Biosensors · 2022Review
- Ultracompact fluorescence smartphone attachment using built-in optics for protoporphyrin-IX quantification in skin.Biomedical optics express · 2021Article
- Single antibody detection in a DNA origami nanoantenna.iScience · 2021Article
- Cellphone enabled point-of-care assessment of breast tumor cytology and molecular HER2 expression from fine-needle aspirates.NPJ breast cancer · 2021Article
- DNA Origami-Enabled Plasmonic Sensing.The journal of physical chemistry. C, Nanomaterials and interfaces · 2021Review
- Rapid, user-friendly, and inexpensive detection of azidothymidine.Analytical and bioanalytical chemistry · 2021Article
- Self-Regeneration and Self-Healing in DNA Origami Nanostructures.Angewandte Chemie (International ed. in English) · 2021Article
- Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope.Nature communications · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Smartphone-based fluorescence microscopy has been rapidly developing over the last few years, enabling point-of-need detection of cells, bacteria, viruses, and biomarkers. These mobile microscopy devices are cost-effective, field-portable, and easy to use, and benefit from economies of scale. Recent developments in smartphone camera technology have improved their performance, getting closer to that of lab microscopes. Here, we report the use of DNA origami nanobeads with predefined numbers of fluorophores to quantify the sensitivity of a smartphone-based fluorescence microscope in terms of the minimum number of detectable molecules per diffraction-limited spot. With the brightness of a single dye molecule as a reference, we compare the performance of color and monochrome sensors embedded in state-of-the-art smartphones. Our results show that the monochrome sensor of a smartphone can achieve better sensitivity, with a detection limit of ∼10 fluorophores per spot. The use of DNA origami nanobeads to quantify the minimum number of detectable molecules of a sensor is broadly applicable to evaluate the sensitivity of various optical instruments.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.