Evidence map›Paper›PMID 30775094›Full record

ArticleBiomedical optics express2019

Depth-extended, high-resolution fluorescence microscopy: whole-cell imaging with double-ring phase (DRiP) modulation.

Xuanwen Hua, Changliang Guo, Jian Wang, Deborah Kim-Holzapfel, Bryce Schroeder, Wenhao Liu, Junhua Yuan, Jarrod French, Shu Jia

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xuanwen HuaDepartment of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.
Changliang GuoDepartment of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.
Jian WangDepartment of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794, USA.
Deborah Kim-HolzapfelDepartment of Biochemistry and Cell Biology and Department of Chemistry, Stony Brook University, Stony Brook, New York 11794, USA.
Bryce SchroederDepartment of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794, USA.
Wenhao LiuDepartment of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.
Junhua YuanDepartment of Physics, University of Science and Technology of China, Hefei 230026, China.
Jarrod FrenchDepartment of Biochemistry and Cell Biology and Department of Chemistry, Stony Brook University, Stony Brook, New York 11794, USA.
Shu JiaDepartment of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.

Funding

Toward Systems Biophotonics: Imaging Biology across High Dimensions and ScalesR35GM124846 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI Shu Jia · 2017 to 2026
$4.0M
Mechanisms that govern assembly and function of higher order protein structures of purine metabolic enzymesR35GM124898 · NIGMS · UNIVERSITY OF MINNESOTA · PI FRENCH, JARROD B · 2017 to 2021
$2.2M
NIGMS NIH HHS R35 GM124846NIGMS NIH HHS R35 GM124898
6 · The paper itself

Abstract

We report a depth-extended, high-resolution fluorescence microscopy system based on interfering Bessel beams generated with double-ring phase (DRiP) modulation. The DRiP method effectively suppresses the Bessel side lobes, exhibiting a high resolution of the main lobe throughout a four- to five-fold improved depth of focus (DOF), compared to conventional wide-field microscopy. We showed both theoretically and experimentally the generation and propagation of a DRiP point-spread function (DRiP-PSF) of the imaging system. We further developed an approach for creating an axially-uniform DRiP-PSF and successfully demonstrated diffraction-limited, depth-extended imaging of cellular structures. We expect the DRiP method to contribute to the fast-developing field of non-diffracting-beam-enabled optical microscopy and be useful for various types of imaging modalities.

Identifiers

PMID30775094
PMCPMC6363204

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.