Evidence map›Paper›PMID 30773767›Full record

ArticleAngewandte Chemie (International ed. in English)2019

A Photo-responsive Small-Molecule Approach for the Opto-epigenetic Modulation of DNA Methylation.

Ha Phuong Nguyen, Sabrina Stewart, Mikiembo N Kukwikila, Sioned Fôn Jones, Daniel Offenbartl-Stiegert, Shiqing Mao, Shankar Balasubramanian, Stephan Beck, Stefan Howorka

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.2field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 17 citations in OpenAlex.

  1. Control of Gemcitabine Activity With Blue and Red Light.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026
    Article
  2. Article
  3. Article
  4. Light-Activation of DNA-Methyltransferases.Angewandte Chemie (International ed. in English) · 2021
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Ha Phuong NguyenDepartment of Chemistry, Institute for Structural and Molecular Biology, University College London, 20 Gordon Street, London, WC1H 0AJ, UK.
Sabrina StewartUCL Cancer Institute, London, UK.
Mikiembo N KukwikilaDepartment of Chemistry, Institute for Structural and Molecular Biology, University College London, 20 Gordon Street, London, WC1H 0AJ, UK.
Sioned Fôn JonesDepartment of Chemistry, Institute for Structural and Molecular Biology, University College London, 20 Gordon Street, London, WC1H 0AJ, UK.
Daniel Offenbartl-StiegertDepartment of Chemistry, Institute for Structural and Molecular Biology, University College London, 20 Gordon Street, London, WC1H 0AJ, UK.
Shiqing MaoDepartment of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK.
Shankar BalasubramanianDepartment of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK.
Stephan BeckUCL Cancer Institute, London, UK.
Stefan HoworkaDepartment of Chemistry, Institute for Structural and Molecular Biology, University College London, 20 Gordon Street, London, WC1H 0AJ, UK.ORCID 0000-0002-6527-2846
Institute of Structural and Molecular Biology · GBCRUK Lung Cancer Centre of Excellence · GBUniversity of Cambridge · GB

Funding

PHOSPHORYLATION OF TRANSCRIPTION FACTORS BY PP90RSKF32CA009282 · NCI · HARVARD UNIVERSITY · PI BROTT, BARBARA K · 1992 to 1995
–
Biotechnology and Biological Sciences Research Council BB/M025373/1Biotechnology and Biological Sciences Research Council BB/N017331/1Cancer Research UK C14303/A17197Engineering and Physical Sciences Research Council EP/N009282/1Medical Research Council G1000411Royal Society WM100023Wellcome TrustWellcome Trust 099232/z/12/zWellcome Trust 84071
6 · The paper itself

Abstract

Controlling the functional dynamics of DNA within living cells is essential in biomedical research. Epigenetic modifications such as DNA methylation play a key role in this endeavour. DNA methylation can be controlled by genetic means. Yet there are few chemical tools available for the spatial and temporal modulation of this modification. Herein, we present a small-molecule approach to modulate DNA methylation with light. The strategy uses a photo-tuneable version of a clinically used drug (5-aza-2'-deoxycytidine) to alter the catalytic activity of DNA methyltransferases, the enzymes that methylate DNA. After uptake by cells, the photo-regulated molecule can be light-controlled to reduce genome-wide DNA methylation levels in proliferating cells. The chemical tool complements genetic, biochemical, and pharmacological approaches to study the role of DNA methylation in biology and medicine.

Indexed as

DNA MethylationEpigenesis, GeneticHumanscytosineDNAepigeneticsmethylationphoto-caging

Identifiers

PMID30773767
PMCPMC7027477
OpenAlexW2915479238

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.