ArticleCell chemical biology2019
Detection of Low-Abundance Metabolites in Live Cells Using an RNA Integrator.
Article in Cell chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- De novo-designed ribozyme-controlled riboregulator for cell-free diagnostics.Nature communications · 2026Article
- Programmable fluorescent aptamer-based RNA switches for rapid identification of point mutations.Nature chemistry · 2025Article
- Cell-free RNAs in maternal peripheral blood as potential biomarkers of preeclampsia: a review.Reproductive biology and endocrinology : RB&E · 2025Review
- Quencher-Free Fluorescence Monitoring of G-Quadruplex Folding.ACS omega · 2025Article
- Artificial dynamic structure ensemble-guided rational design of a universal RNA aptamer-based sensing tag.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Fluorogenic RNA-Based Biosensors of Small Molecules: Current Developments, Uses, and Perspectives.Biosensors · 2024Review
- Article
- An RNA Motif That Enables Optozyme Control and Light-Dependent Gene Expression in Bacteria and Mammalian Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Genetically encoded RNA-based sensors with Pepper fluorogenic aptamer.Nucleic acids research · 2023Article
- Real-Time Assessment of Intracellular Metabolites in Single Cells through RNA-Based Sensors.Biomolecules · 2023Review
- Ribocentre: a database of ribozymes.Nucleic acids research · 2023Article
- Assembly of long L-RNA by native RNA ligation.Chemical communications (Cambridge, England) · 2021Article
- Review
- Considerations for using isolated cell systems to understand cardiac metabolism and biology.Journal of molecular and cellular cardiology · 2021Review
- Guanidine Biosensors Enable Comparison of Cellular Turn-on Kinetics of Riboswitch-Based Biosensor and Reporter.ACS synthetic biology · 2021Article
- Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules.Analytical methods : advancing methods and applications · 2020Article
- RNA-based fluorescent biosensors for live cell imaging of small molecules and RNAs.Current opinion in biotechnology · 2020Review
- Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase-Modified Fluorogenic RNA Aptamers.Angewandte Chemie (International ed. in English) · 2020Article
- Structure-fluorescence activation relationships of a large Stokes shift fluorogenic RNA aptamer.Nucleic acids research · 2019Article
- Self-powered RNA nanomachine driven by metastable structure.Nucleic acids research · 2019Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Genetically encoded biosensors are useful tools for detecting the presence and levels of diverse biomolecules in living cells. However, low-abundance targets are difficult to detect because they are often unable to bind and activate enough biosensors to detect using standard microscopic imaging approaches. Here we describe a type of RNA-based biosensor, an RNA integrator, which enables detection of low-abundance targets in vitro and in living cells. The RNA integrator is an RNA sequence comprising a ribozyme and an unfolded form of the fluorogenic aptamer Broccoli. Upon binding its target, the ribozyme undergoes cleavage and releases Broccoli, which subsequently folds and becomes fluorescent. Importantly, each target molecule can bind and induce cleavage of multiple copies of the integrator sensor, resulting in an amplified signal. We show that this approach can be generalized to numerous different ribozyme types for the detection of various small molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.