ArticleBiophysical journal2019
High-Copy-Number Plasmid Segregation-Single-Molecule Dynamics in Single Cells.
Article in Biophysical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- ProFiT-SPEci-FISH: a novel approach for linking plasmids to hosts in complex microbial communities at the single-cell level.Microbiome · 2025Article
- Dynamic plasmid clustering in Bacteria: Influence of transcriptional activity and host cellular states.Biochemistry and biophysics reports · 2025Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth ineLife · 2025Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth inbioRxiv : the preprint server for biology · 2025Article
- Spatial mapping of mobile genetic elements and their bacterial hosts in complex microbiomes.Nature microbiology · 2024Article
- Spatial Mapping of Mobile Genetic Elements and their Cognate Hosts in Complex Microbiomes.bioRxiv : the preprint server for biology · 2023Article
- Confinement anisotropy drives polar organization of two DNA molecules interacting in a nanoscale cavity.Nature communications · 2022Article
- Segregational Drift Constrains the Evolutionary Rate of Prokaryotic Plasmids.Molecular biology and evolution · 2021Article
- Construction of intracellular asymmetry and asymmetric division in Escherichia coli.Nature communications · 2021Article
- Mathematical Models of Plasmid Population Dynamics.Frontiers in microbiology · 2021Review
- A High-Resolution View of Adaptive Event Dynamics in a Plasmid.Genome biology and evolution · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial high-copy-number (hcn) plasmids provide an excellent model to study the underlying physical mechanisms of DNA segment segregation in an intracellular context. Using two-color fluorescent repressor-operator systems and a synthetic repressible replication origin, we tracked the motion and segregation of single hcn plasmid molecules in individual cells. The plasmid diffusion dynamics revealed between-plasmid temporal associations (clustering) as well as entropic and elastic recoiling forces in the confined intracellular spaces outside of nucleoids. These two effects could be effectively used in models to predict the heterogeneity of segregation. Additionally, the motile behaviors of hcn plasmids provide quantitative estimates of entropic exclusion strength and dynamic associations between DNA segments. Overall, this study utilizes a, to our knowledge, novel approach to predict the polymer dynamics of DNA segments in spatially confined, crowded cellular compartments as well as during bacterial chromosome segregation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.