Evidence map›Paper›PMID 30771096›Full record

ArticleMikrochimica acta2019

Determination of alkaline phosphatase activity based on enzyme-triggered generation of a thiol and the fluorescence quenching of silver nanoclusters.

Minchuan Luo, Zhu Su, Xinyi Wang, Liang Li, Yifeng Tu, Jilin Yan

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Minchuan LuoThe Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou, 215123, China.
Zhu SuThe Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou, 215123, China.
Xinyi WangThe Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou, 215123, China.
Liang LiSchool of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan, 430205, China.
Yifeng TuThe Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou, 215123, China.
Jilin YanThe Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou, 215123, China. yanjl@suda.edu.cn.
Soochow University · CNWuhan Institute of Technology · CN

Funding

Priority Academic Program Development of Jiangsu Higher Education Institutions PAPDProject of Scientific and Technologic Infrastructure of Suzhou SZS201708Young Scientists Fund 21305100
6 · The paper itself

Abstract

A fluorimetric method is described for the determination of alkaline phosphatase (ALP) activity. It is based on the use of polyethyleneimine-coated silver nanoclusters (AgNCs), which display an intense blue fluorescence peaking at 450 nm (under 375 nm excitation). ALP catalyzes the dephosphorylation of the thiophosphate amifostine to generate a thiol that binds to the AgNCs and causes its fluorescence to be quenched. Under the optimal experimental conditions, fluorescence linearly drops in the 0.08-2.0 U L

Indexed as

Alkaline PhosphataseAmifostineEnzyme AssaysFluorescenceFluorometryHumansLimit of DetectionMetal NanoparticlesPolyethyleneimineSilverSulfhydryl CompoundsUltraviolet RaysAlkaline PhosphataseAmifostinePolyethyleneimineSilverSulfhydryl CompoundsAggregationDephosphorylationFluorimetric methodSerum samplesSilver nanoparticlesThiophosphate

Identifiers

PMID30771096
OpenAlexW2913872022

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.