Evidence map›Paper›PMID 30771006›Full record

ArticleMikrochimica acta2019

Electrochemiluminescent determination of the activity of uracil-DNA glycosylase: Combining nicking enzyme assisted signal amplification and catalyzed hairpin assembly.

Qiao Liu, Chao Liu, Guang Zhu, Haifeng Xu, Xiao Jie Zhang, Chongju Hu, Yong Xie, Keying Zhang, Hongyan Wang

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 21 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Qiao LiuAnhui Key Laboratory of Spin Electron and Nanomaterials, School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 234000, People's Republic of China.ORCID http://orcid.org/0000-0003-3001-8482
Chao LiuAnhui Key Laboratory of Spin Electron and Nanomaterials, School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 234000, People's Republic of China.
Guang ZhuAnhui Key Laboratory of Spin Electron and Nanomaterials, School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 234000, People's Republic of China.
Haifeng XuAnhui Key Laboratory of Spin Electron and Nanomaterials, School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 234000, People's Republic of China.
Xiao Jie ZhangAnhui Key Laboratory of Spin Electron and Nanomaterials, School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 234000, People's Republic of China.
Chongju HuAnhui Key Laboratory of Spin Electron and Nanomaterials, School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 234000, People's Republic of China.
Yong XieAnhui Key Laboratory of Spin Electron and Nanomaterials, School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 234000, People's Republic of China.
Keying ZhangAnhui Key Laboratory of Spin Electron and Nanomaterials, School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 234000, People's Republic of China. zhangky1983@163.com.
Hongyan WangAnhui Key Laboratory of Spin Electron and Nanomaterials, School of Chemistry and Chemical Engineering, Suzhou University, Suzhou, 234000, People's Republic of China. suzhouwhy@163.com.
Suzhou University of Science and Technology · CN

Funding

University Natural Science Research Project of Anhui Province No. 2016SCXPTTD
6 · The paper itself

Abstract

An electrochemiluminescence (ECL) based method is described for the determination of the activity of the enzyme uracil-DNA glycosylase (UDG). It is based on the use of nicking enzyme-assisted signal amplification and catalytic hairpin assembly. UDG can recognize and hydrolyze the uracil bases from the stem of hairpin DNA1 (HP1). This causes the opening of HP1 to form a straight strand DNA. The straight HP1 can hybridize with hairpin DNA2 (HP2) to form a DNA duplex. In the presence of nicking enzyme, it can recognize and cut the specific sequences in the HP2 of the DNA duplex, and a subsequent release of HP1. It hybridizes with other HP2 to trigger the continuous cleavage of HP2, concomitantly generating abundant intermediate sequences (S1). The hairpin DNA3 (HP3) is immobilized on a gold electrode via Au-S chemistry. In the presence of S1, HP3 hybridizes with S1 and its hairpin structure is opened. This hybridization causes displacement from hairpin DNA4 (HP4), and S1 is released to initiate the next hybridization process. Thus, a massive number of HP3-HP4 duplexes is generated after the cyclic process. Subsequently, the cDNA modified on bio-bar-coded AuNP-CdSe quantum dots are immobilized on the electrode by hybridization with the redundant part of the opened HP4. This results in a significant amplification of the ECL signal. This biosensor is sensitive and selective for UDG. The detection limit is 6 mU·mL

Indexed as

Base SequenceBiosensing TechniquesDeoxyribonuclease IDeoxyribonucleases, Type II Site-SpecificDNAElectrochemical TechniquesEnzyme AssaysGoldInverted Repeat SequencesLimit of DetectionLuminescenceLuminescent MeasurementsMetal NanoparticlesNucleic Acid Amplification TechniquesNucleic Acid HybridizationQuantum DotsDeoxyribonuclease IDeoxyribonucleases, Type II Site-SpecificDNAGoldUracil-DNA GlycosidaseBiosensorCatalyzed hairpin assemblyElectrochemiluminescenceSignal amplificationUracil-DNA glycosylase

Identifiers

PMID30771006
OpenAlexW2911663872

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.