ArticleScientific reports2019
A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Nanotechnology Strategies in Plant Genetic Engineering: Intelligent Delivery and Precision Editing.Plants (Basel, Switzerland) · 2025Review
- Electroporation-Mediated Gene Delivery in Pyropia yezoensis (Rhodophyta) Without Cell Wall Removal.Marine biotechnology (New York, N.Y.) · 2025Article
- Direct Nuclear Delivery of Proteins on Living Plant via Partial Enzymatic Cell Wall Digestion.Current issues in molecular biology · 2024Article
- Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species.Plant cell reports · 2024Review
- Direct protein delivery into intact Arabidopsis cells for genome engineering.Scientific reports · 2024Article
- Penetrating the ultra-tough yeast cell wall with finite element analysis model-aided design of microtools.iScience · 2024Article
- Article
- Recent Progress of Rational Modified Nanocarriers for Cytosolic Protein Delivery.Pharmaceutics · 2023Review
- A Method for Electroporation of Cre Recombinase Protein into IntactPlants (Basel, Switzerland) · 2023Article
- Shoot-root signal circuit: Phytoremediation of heavy metal contaminated soil.Frontiers in plant science · 2023Review
- Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration.Frontiers in genome editing · 2023Review
- TAQing2.0 for genome reorganization of asexual industrial yeasts by direct protein transfection.Communications biology · 2022Article
- Microneedle Array-Assisted, Direct Delivery of Genome-Editing Proteins Into Plant Tissue.Frontiers in plant science · 2022Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Nanoparticles for protein delivery in planta.Current opinion in plant biology · 2021Review
- Preculture in an enriched nutrient medium greatly enhances thePlant biotechnology (Tokyo, Japan) · 2021Article
- Gene transfer to plants by electroporation: methods and applications.Molecular biology reports · 2020Review
- Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system.PloS one · 2020Article
- Article
- Genome Editing in Agriculture: Technical and Practical Considerations.International journal of molecular sciences · 2019Review
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome engineering in plants is highly dependent on the availability of effective molecular techniques. Despite vast quantities of research, genome engineering in plants is still limited in terms of gene delivery, which requires the use of infectious bacteria or harsh conditions owing to the difficulty delivering biomaterial into plant cells through the cell wall. Here, we describe a method that uses electroporation-mediated protein delivery into cultured Arabidopsis thaliana cells possessing an intact cell wall, and demonstrate Cre-mediated site-specific recombination. By optimizing conditions for the electric pulse, protein concentration, and electroporation buffer, we were able to achieve efficient and less-toxic protein delivery into Arabidopsis thaliana cells with 83% efficiency despite the cell wall. To the best of our knowledge, this is the first report demonstrating the electroporation-mediated protein delivery of Cre recombinase to achieve nucleic acid-free genome engineering in plant cells possessing an intact cell wall.
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