ArticleProceedings of the National Academy of Sciences of the United States of America2019
Nanometer-accuracy distance measurements between fluorophores at the single-molecule level.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Spontaneous and indefinite blinking in upconverting nanoparticles for ångström-precision multicolour super-resolution imaging.Nature nanotechnology · 2026Article
- Uninterrupted optical resolution of identical point scatterers undergoing nanometric changes in distance.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- NanorulerQA: quantitative quality analysis of dual-color DNA nanorulers via single-molecule photobleaching step counting and spatio-temporal colocalization.Biomedical optics express · 2026Article
- A methodology to reduce the localization error in multi-loci microscopy provides new insights into enhancer biology.PLoS computational biology · 2025Article
- Pair cross-correlation analysis for assessing protein co-localization.Biophysical journal · 2025Article
- Identifying intermolecular interactions in single-molecule localization microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Multicolor Tracking of Molecular Motors at Nanometer Resolution.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Simultaneous multicolor fluorescence imaging using PSF splitting.Nature methods · 2024Article
- PyF2F: a robust and simplified fluorophore-to-fluorophore distance measurement tool for Protein interactions from Imaging Complexes after Translocation experiments.NAR genomics and bioinformatics · 2024Article
- Tolerance to aberration and misalignment in a two-point-resolving image inversion interferometer.Optics express · 2023Article
- Ubiquitin modulates 26Science advances · 2022Article
- Bayesian inference of multi-point macromolecular architecture mixtures at nanometre resolution.PLoS computational biology · 2022Article
- Choosing the Probe for Single-Molecule Fluorescence Microscopy.International journal of molecular sciences · 2022Review
- Extraction of accurate cytoskeletal actin velocity distributions from noisy measurements.Nature communications · 2022Article
- Estimating the localization spread function of static single-molecule localization microscopy images.Biophysical journal · 2022Article
- Three-color single-molecule imaging reveals conformational dynamics of dynein undergoing motility.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Microscope calibration protocol for single-molecule microscopy.Optics express · 2021Article
- Molecular height measurement by cell surface optical profilometry (CSOP).Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells.Science advances · 2020Article
- Coupling of ATPase activity, microtubule binding, and mechanics in the dynein motor domain.The EMBO journal · 2019Article
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6 authors.
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Abstract
Light microscopy is a powerful tool for probing the conformations of molecular machines at the single-molecule level. Single-molecule Förster resonance energy transfer can measure intramolecular distance changes of single molecules in the range of 2 to 8 nm. However, current superresolution measurements become error-prone below 25 nm. Thus, new single-molecule methods are needed for measuring distances in the 8- to 25-nm range. Here, we describe methods that utilize information about localization and imaging errors to measure distances between two different color fluorophores with ∼1-nm accuracy at distances >2 nm. These techniques can be implemented in high throughput using a standard total internal reflection fluorescence microscope and open-source software. We applied our two-color localization method to uncover an unexpected ∼4-nm nucleotide-dependent conformational change in the coiled-coil "stalk" of the motor protein dynein. We anticipate that these methods will be useful for high-accuracy distance measurements of single molecules over a wide range of length scales.
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