ArticlePoultry science2019
Effects of protease and phytase supplements on small intestinal microbiota and amino acid digestibility in broiler chickens.
Article in Poultry science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 71 citations in OpenAlex.
- Effects of phytase and protease supplementation, with and without considering their nutritional matrix values, on the productive performance of laying hens during the late‑lay period.Veterinary and animal science · 2026Article
- Protease and Phytase Alleviate Digestive Limitations of Canola Seed in Broiler Chickens.Veterinary medicine and science · 2026Article
- Effects of protease and lipase supplementation in starter diet on growth performance, small intestine morphology, and immunity in young broilers.Veterinary and animal science · 2026Article
- Novel Insights into Metagenomic-Assembled Genomes from Layer Chicken Housing Environment.International journal of molecular sciences · 2026Article
- Multi-protease supplementation enhances growth performance and promotes gut health in broilers fed low-protein, low-amino acid diets.Translational animal science · 2026Article
- Can dietary protease ameliorate performance and gut integrity in broilers fed on a diet low in digestible lysine?Poultry science · 2025Article
- Effect of microencapsulated organic acids-essential oils blend and protease on performance and gut health of broilers under nutritional challenges.Journal of animal science and technology · 2025Article
- Effects of exogenous protease supplementation of diets containing animal proteins or not on Campylobacter jejuni colonization and on the intestinal microbiota of broiler chickens.Gut pathogens · 2025Article
- Do we need a standardized 16S rRNA gene amplicon sequencing analysis protocol for poultry microbiota research?Poultry science · 2025Review
- Protease andAnimals : an open access journal from MDPI · 2025Article
- Effects of xylanase and phytase supplementation in diets containingFrontiers in veterinary science · 2025Article
- Unlocking Phytate with Phytase: A Meta-Analytic View of Meat-Type Chicken Muscle Growth and Bone Mineralization Potential.Animals : an open access journal from MDPI · 2024Review
- Effects of high dietary inclusion of Arthrospira platensis, either extruded or supplemented with a super-dosing multi-enzyme mixture, on broiler growth performance and major meat quality parameters.BMC veterinary research · 2024Article
- Comparative effects of proteases on performance, carcass traits and gut structure of broilers fed diets reduced in protein and amino acids.Journal of animal science and technology · 2024Article
- Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels.Poultry science · 2024Article
- Partial replacement of soybean with local alternative sources: effects on behavior, cecal microbiota, and intestinal histomorphometry of local chickens.Frontiers in veterinary science · 2024Article
- Effect of an Escherichia coli-derived phytase and a carbohydrase-protease cocktail derived from Bacillus spp. on performance, digestibility, bone mineralization and gut morphology in broilers fed different nutrient density diets.Veterinary medicine and science · 2024Article
- Effects of Different Doses of Multienzyme Supplementation on Growth Performance, Duodenal pH and Morphology, and Carcass Traits in Broilers Fed Diets with an Increasing Reduction in Energy.Animals : an open access journal from MDPI · 2023Article
- Effects of Different Proteases on Protein Digestion In Vitro and In Vivo and Growth Performance of Broilers Fed Corn-Soybean Meal Diets.Animals : an open access journal from MDPI · 2023Article
- Effect of exogenus protease on performance, nutrient digestibility, intestinal histomorphometric, meat quality characteristics, carcass yield in broilers fed low protein diets.Tropical animal health and production · 2023Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to determine the effects of protease origin and dosage on the prececal (pc) amino acid (AA) digestibility and the influence on composition of the microbial community in the small intestine. In addition, the effects of phytase supplementation were investigated. A total of 8 dietary treatments were included. The basal diet contained mainly corn and soybean meal. Three protease products were added to the basal diet, each at the level recommended by the supplier and at an 8-fold level. Phytase was supplemented in another dietary treatment. Each dietary treatment was allocated to 8 replicates of 15 birds each. The experimental diets were offered from day 15 to 21 for ad libitum consumption. The effect of protease supplementation on the pc AA digestibility depended on the protease product type and the amount supplemented. The pc AA digestibility was significantly increased by 1 protease product when supplemented at high level and when phytase was supplemented. In all the other treatments, protease supplementation had no significant influence or it decreased pc AA digestibility, when compared with the treatment with no enzymes added. In general, Firmicutes was the most abundant phylum among the ileal microbiota across all the treatments. Significant effects on microbiota composition were observed at the genus level for some but not all protease treatments and phytase supplementation. The genera Streptococcus, Lactobacillus, and uncultured Clostridiaceae were responsible for these differences. Furthermore, microbial networks established for each diet showed either high or low number of intergeneric interactions, but without a consistent enzyme effect. We conclude that enzyme supplementation effects were evident in the terminal small intestine microbiota composition, and to a lesser extent, in pc AA digestibility. However, the changes in microbiota composition and pc AA digestibility could not be correlated, indicating absence of a causal relationship.
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Registered trials
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