Evidence map›Paper›PMID 30767768›Full record

ArticleMalaria journal2019

Updating the modified Thompson test by using whole-body bioluminescence imaging to replace traditional efficacy testing in experimental models of murine malaria.

Diana Caridha, Mark Hickman, Lisa Xie, Franklyn Ngundam, Erin Milner, Amanda Schenk, Kirk Butler, Dylan Nugent, Patricia Lee, Norma Roncal and 9 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Malaria journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
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  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 4 institutions in 1 country.

Diana CaridhaWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA. diana.caridha2.ctr@mail.mil.ORCID http://orcid.org/0000-0002-9857-0719
Mark HickmanBiodefense Therapeutics, JPdM-BDTx, 110 Thomas Johnson Dr., Frederick, MD, USA.
Lisa XieWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Franklyn NgundamWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Erin MilnerDwight David Eisenhower Army Medical Center, 300 E Hospital Rd, Ft Gordon, GA, 30905, USA.
Amanda SchenkWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Kirk ButlerWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Dylan NugentWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Patricia LeeWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Norma RoncalWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Susan LeedWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Eve HosfordWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Jangwoo LeeWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Richard J SciottiWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Gregory ReichardWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Chad BlackWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Mara Kreishman-DeitrickWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Qigui LiWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Brian VeselyWalter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
Walter Reed Army Institute of Research · USEisenhower Medical Center · USIo Therapeutics (United States) · USNational Institutes of Health · US

Funding

Military Infectious Disease Research Program (MIDRP) Area Q - Anti-parasitic drugsMilitary Infectious Disease Research Program (MIDRP) proposal #Q0433_15_WR_CS_OC.
6 · The paper itself

Abstract

backgroundRodent malaria models are extensively used to predict treatment outcomes in human infections. There is a constant need to improve and refine these models by innovating ways to apply new scientific findings and cutting edge technologies. In addition, and in accordance with the three R's of animal use in research, in vivo studies should be constantly refined to avoid unnecessary pain and distress to the experimental animals by using preemptive euthanasia as soon as the main scientific study objective has been accomplished.

methodsThe new methodology described in this manuscript uses the whole-body bioluminescence signal emitted by transgenic, luciferase-expressing Plasmodium berghei parasites to assess the parasite load predicted parasitaemia (PLPP) in drug and control treated female ICR-CD1 mice infected with 1 × 10

resultsThere is a good correlation between whole-body bioluminescence signal and parasitaemia measured using Giemsa-stained thin blood smears and flow cytometry respectively in donor and study mice in the modified Thompson test. The algebraic formulas which represent these correlations can be successfully used to assess PLPP in donor and study mice. In addition, the new methodology can pinpoint sick animals 2-8 days before they would have been otherwise diagnosed based on behavioural or any other signs of malaria disease.

conclusionsThe new method for predicting parasitaemia in the modified Thompson test is simple, precise, objective, and minimizes false positive results that can lead to the premature removal of animals from study. Furthermore, from the animal welfare perspective of replace, reduce, and refine, this new method facilitates early removal of sick animals from study as soon as the study objective has been achieved, in many cases well before the clinical signs of disease are present.

Indexed as

Disease Models, AnimalParasite LoadAnimalsAntimalarialsFemaleGenes, ReporterHumansLuminescent MeasurementsMalariaMice, Inbred ICRParasitemiaPlasmodium bergheiStaining and LabelingTreatment OutcomeWhole Body ImagingAntimalarialsAnimal modelsAnti-malarial drugsBioluminescenceIn vivo imaging technologyParasitaemia readoutParasite load predicted parasitaemia (PLPP)Plasmodium bergheiPreemptive euthanasiaRefinement

Identifiers

PMID30767768
PMCPMC6376706
OpenAlexW2921209895

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.