ArticleChembiochem : a European journal of chemical biology2019
A Concise, Modular Antibody-Oligonucleotide Conjugation Strategy Based on Disuccinimidyl Ester Activation Chemistry.
Article in Chembiochem : a European journal of chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Antibody-oligonucleotide conjugates: an emerging modality for precision RNA therapeutics.Antibody therapeutics · 2026Review
- Small Interfering RNA Carriers for Oncotherapy: A Preclinical Overview.Pharmaceutics · 2025Review
- Single-cell chemoproteomics identifies metastatic activity signatures in breast cancer.Science advances · 2024Article
- iSECRETE: Integrating Microfluidics and DNA Proximity Amplification for Synchronous Single-Cell Activation and IFN-γ Secretion Profiling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Selection of antibody-binding covalent aptamers.Communications chemistry · 2024Article
- Therapeutic Oligonucleotides: An Outlook on Chemical Strategies to Improve Endosomal Trafficking.Cells · 2023Review
- Spatial Chemoproteomics for Mapping the Active Proteome.Israel journal of chemistry · 2023Article
- Site-specific DNA functionalization through the tetrazene-forming reaction in ionic liquids.Chemical science · 2022Article
- A Platform for Site-Specific DNA-Antibody Bioconjugation by Using Benzoylacrylic-Labelled Oligonucleotides.Angewandte Chemie (International ed. in English) · 2021Article
- Convertible and Constrained Nucleotides: The 2'-Deoxyribose 5'-C-Functionalization Approach, a French Touch.Molecules (Basel, Switzerland) · 2021Review
- Antibody-Oligonucleotide Conjugates: A Twist to Antibody-Drug Conjugates.Journal of clinical medicine · 2021Review
- In Vivo Imaging of the Tumor-Associated Enzyme NCEH1 with a Covalent PET Probe.Angewandte Chemie (International ed. in English) · 2020Article
- Ultrasensitive, multiplexed chemoproteomic profiling with soluble activity-dependent proximity ligation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Chemical probes for spatially resolved measurement of active enzymes in single cells.Methods in enzymology · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The synthesis of antibody-oligonucleotide conjugates has enabled the development of highly sensitive bioassays for specific epitopes in the laboratory and clinic. Most synthetic schemes to generate these hybrid molecules require expensive reagents, significant quantities of input antibody, and multistep purification routes; thus limiting widespread application. Herein a facile and robust conjugation strategy is reported that involves "plug-and-play" antibody conjugation with succinimidyl-functionalized oligonucleotides, which are high yielding and compatible for use directly after buffer exchange. The succinimidyl-linked oligonucleotides are synthesized with 5'-amine-modified oligonucleotides and disuccinimidyl suberate (DSS), both of which are inexpensive and commercially available. Direct incubation of the resulting stable succinimidyl- oligonucleotide conjugates with commercial antibodies yields conjugates ready for use after benchtop buffer exchange. It is demonstrated that the resulting oligonucleotide-antibody and oligonucleotide-streptavidin conjugates retain potent and specific binding in activity-dependent proximity ligation imaging, and proximity ligation-mediated qPCR detection of endogenous proteins in native cellular contexts down to picogram levels of whole proteome. This DSS conjugation strategy should be widely applicable in the synthesis of protein-oligonucleotide conjugates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.