Evidence map›Paper›PMID 30766915›Full record

ArticleAdvanced biosystems2018

Evaluation of the topographical influence on the cellular behavior of human umbilical vein endothelial cells.

Marek Kukumberg, Yuan Yao, Seok Hong Goh, Dawn Jh Neo, Jia Yi Yao, Evelyn Kf Yim

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced biosystems, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Marek KukumbergMechanobiology Institute, National University of Singapore, #05-01 T-lab, 5A Engineering Drive 1, Singapore 117411.
Yuan YaoDepartment of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Seok Hong GohInstitute of Materials Research and Engineering, Agency for Science, Technology and Research (ASTAR), 2 Fusionopolis Way, Innovis, 138634, Singapore, Department of Biomedical Engineering, National University of Singapore, E4, #04-10,4 Engineering Drive 3, Singapore 117583.
Dawn Jh NeoMechanobiology Institute, National University of Singapore, #05-01 T-lab, 5A Engineering Drive 1, Singapore 117411.
Jia Yi YaoDepartment of Biomedical Engineering, National University of Singapore, E4, #04-10,4 Engineering Drive 3, Singapore 117583.
Evelyn Kf YimMechanobiology Institute, National University of Singapore, #05-01 T-lab, 5A Engineering Drive 1, Singapore 117411, Department of Biomedical Engineering, National University of Singapore, E4, #04-10,4 Engineering Drive 3, Singapore 117583, Department of Surgery, National University of Singapore, NUHS Tower Block, Level 8,1E Kent Ridge Road, Singapore 119228, Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.

Funding

Surface Modification Compliant Vascular GraftsR01HL130274 · NHLBI · OREGON HEALTH & SCIENCE UNIVERSITY · PI HINDS, MONICA T, YIM, EVELYN KING FAI · 2016 to 2019
$2.6M
NHLBI NIH HHS R01 HL130274
6 · The paper itself

Abstract

Adhesion and proliferation of vascular endothelial cells are important parameters in the endothelialization of biomedical devices for vascular applications. Endothelialization is a complex process affected by endothelial cells and their interaction with the extracellular microenvironment. Although numerous approaches are taken to study the influence of the external environment, a systematic investigation of the impact of an engineered microenvironment on endothelial cell processes is needed. This study aims to investigate the influence of topography, initial cell seeding density, and collagen coating on human umbilical vein endothelial cells (HUVECs). Utilizing the MultiARChitecture (MARC) chamber, the effects of various topographies on HUVECs are identified, and those with more prominent effects were further evaluated individually using the MARC plate. Endothelial cell marker expression and monocyte adhesion assay are examined on the HUVEC monolayer. HUVECs on 1.8 μm convex and concave microlens topographies demonstrate the lowest cell adhesion and proliferation, regardless of initial cell seeding density and collagen I coating, and the HUVEC monolayer on the microlens shows the lowest monocyte adhesion. This property of lens topographies would potentially be a useful parameter in designing vascular biomedical devices. The MARC chamber and MARC plate show a great potential for faster and easy pattern identification for various cellular processes.

Indexed as

endothelial cellsextracellular matrix coatinghigh throughput screeningmicrotopographymonocytes adhesion assayproliferation

Identifiers

PMID30766915
PMCPMC6370334

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.