Evidence map›Paper›PMID 30766897›Full record

ArticleRegenerative therapy2019

Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds.

Yasuhiko Kaita, Takehiko Tarui, Hideaki Yoshino, Takeaki Matsuda, Yoshihiro Yamaguchi, Takatoshi Nakagawa, Michio Asahi, Masaaki Ii

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 5 pooled it
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 5 syntheses or guidelines pooled it, 30 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
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  4. Pooled it
  5. Pooled it
  6. Review
  7. Supercooled preservation of cultured primary rat hepatocyte monolayers.Frontiers in bioengineering and biotechnology · 2024
    Article
  8. Review
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  13. Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2020
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Yasuhiko KaitaDepartment of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, Japan.
Takehiko TaruiDepartment of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, Japan.
Hideaki YoshinoDivision of Cardiology, Second Department of Internal Medicine, Kyorin University, School of Medicine, Tokyo, Japan.
Takeaki MatsudaDepartment of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, Japan.
Yoshihiro YamaguchiDepartment of Trauma and Critical Care Medicine, Kyorin University School of Medicine, Tokyo, Japan.
Takatoshi NakagawaDepartment of Pharmacology, Faculty of Medicine, Osaka Medical College, Osaka, Japan.
Michio AsahiDepartment of Pharmacology, Faculty of Medicine, Osaka Medical College, Osaka, Japan.
Masaaki IiDivision of Research Animal Laboratory and Translational Medicine, Research and Development Center, Osaka Medical College, Osaka, Japan.
Kyorin University · JPOsaka Medical and Pharmaceutical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.

methodsFull thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.

resultsIn vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.

conclusionsThese findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

Indexed as

ADRCs, adipose-derived regenerative cellsAdSCs, adipose-derived stem cellsBurnCM, conditioned mediumDAPI, 4′,6-diamidino-2-phenylindoleDMEM-F12, Dulbecco's modified Eagle's medium supplemented with F12EGF, Epidermal growth factorFBS, Fetal bovine serumFGF2, Fibroblast growth factor 2GAPDH, Glyceraldehyde 3-phosphate dehydrogenaseHGF, Hepatocyte growth factorIGF-1, Insulin-like growth factor-1ILB4, Isolectin B4MSC, Mesenchymal stem cellNHDF, Normal human dermal fibroblastNHEK, Normal human epithelial keratinocytePBS, Phosphate-buffered salinePVDF, Poly vinylidene di-fluorideqRT-PCR, Quantitative real-time reverse transcription polymerase chain reactionSDS-PAGE, Sodium dodecyl sulfate-polyacrylamide gel electrophoresisStem cellsSVF, Stromal vascular fractionVEGF, Vascular endothelial growth factorWound healing

Identifiers

PMID30766897
PMCPMC6360408
OpenAlexW2952856505

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.