ArticleFrontiers in neuroanatomy2019
Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain.
Article in Frontiers in neuroanatomy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed.
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Visualising viral interactions and mechanisms at the nanoscale with expansion microscopy.Npj viruses · 2026Review
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- Expanding boundaries - a cell biologist's guide to expansion microscopy.Journal of cell science · 2024Review
- Synergizing Algorithmic Design, Photoclick Chemistry and Multi-Material Volumetric Printing for Accelerating Complex Shape Engineering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Article
- A Model of Discovery: The Role of Imaging Established and Emerging Non-mammalian Models in Neuroscience.Frontiers in molecular neuroscience · 2022Review
- Expansion tomography for large volume tissue imaging with nanoscale resolution.Biomedical optics express · 2021Article
- Protein-retention expansion microscopy for visualizing subcellular organelles in fixed brain tissue.Journal of neuroscience methods · 2021Article
- Comparison of Different Tissue Clearing Methods for Three-Dimensional Reconstruction of Human Brain Cellular Anatomy Using Advanced Imaging Techniques.Frontiers in neuroanatomy · 2021Article
- Application of SNAP-Tag in Expansion Super-Resolution Microscopy Using DNA Oligostrands.Frontiers in chemistry · 2021Article
- Prospects and limitations of expansion microscopy in chromatin ultrastructure determination.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Article
- Fast Retrograde Access to Projection Neuron Circuits Underlying Vocal Learning in Songbirds.Cell reports · 2020Article
- Combining sample expansion and light sheet microscopy for the volumetric imaging of virus-infected cells with super-resolution.Biomedical optics express · 2020Article
- Tissue Clearing and Light Sheet Microscopy: Imaging the Unsectioned Adult Zebra Finch Brain at Cellular Resolution.Frontiers in neuroanatomy · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expansion microscopy and light sheet imaging (ExLSM) provide a viable alternative to existing tissue clearing and large volume imaging approaches. The analysis of intact volumes of brain tissue presents a distinct challenge in neuroscience. Recent advances in tissue clearing and light sheet microscopy have re-addressed this challenge and blossomed into a plethora of protocols with diverse advantages and disadvantages. While refractive index matching achieves near perfect transparency and allows for imaging at large depths, the resolution of cleared brains is usually limited to the micrometer range. Moreover, the often long and harsh tissue clearing protocols hinder preservation of native fluorescence and antigenicity. Here we image large expanded brain volumes of zebra finch brain tissue in commercially available light sheet microscopes. Our expansion light sheet microscopy (ExLSM) approach presents a viable alternative to many clearing and imaging methods because it improves on tissue processing times, fluorophore compatibility, and image resolution.
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Registered trials
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