ArticleScientific reports2019
Ultrasound-assisted extraction optimization and validation of an HPLC-DAD method for the quantification of polyphenols in leaf extracts of Cecropia species.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 31 citations in OpenAlex.
- Evaluation of Aqueous Maceration and Ultrasound-Assisted Extracts ofPharmaceuticals (Basel, Switzerland) · 2025Article
- Bioactive Compounds of Plant-Based Food: Extraction, Isolation, Identification, Characteristics, and Emerging Applications.Food science & nutrition · 2025Review
- Purification of ultrasonic assisted extracted chlorogenic acid fromFood chemistry: X · 2025Article
- Review
- Design of Experiments for Optimizing Ultrasound-Assisted Extraction of Bioactive Compounds from Plant-Based Sources.Molecules (Basel, Switzerland) · 2023Review
- Intra- and Interspecies Differences of TwoPlants (Basel, Switzerland) · 2023Article
- Article
- Study of Phenolic Compounds and Antioxidant Capacity of Spanish Almonds.Foods (Basel, Switzerland) · 2021Article
- Optimization of a Green Ultrasound-Assisted Extraction of Different Polyphenols fromPlants (Basel, Switzerland) · 2020Article
- Integrated Analysis of the Transcriptome and Metabolome ofInternational journal of molecular sciences · 2020Article
- Effect of lipophilic sea buckthorn extract on cream cheese properties.Journal of food science and technology · 2020Article
- Environmentally Friendly Methods for Flavonoid Extraction from Plant Material: Impact of Their Operating Conditions on Yield and Antioxidant Properties.TheScientificWorldJournal · 2020Review
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cecropia species are traditionally used in Latin American folk medicine and are available as food supplements with little information warranting their quality. The optimum conditions for the extraction of chlorogenic acid (CA), total flavonoids (TF) and flavonolignans (FL) from leaves of Cecropia species were determined using a fractional factorial design (FFD) and a central composite design (CCD). A reversed-phase high-performance liquid chromatographic method coupled to a diode array detector (HPLC-DAD) was validated for the quantification of CA, TF and FL, following the ICH guidelines. Quantitative and Principal Component Analysis (PCA) was also performed. The extraction-optimization methodology enabled us developing an appropriate extraction process with a time-efficient execution of experiments. The experimental values agreed with those predicted, thus indicating suitability of the proposed model. The validation parameters for all chemical markers of the quantification method were satisfactory. The results revealed that the method had excellent selectivity, linearity, precision (repeatability and intermediate precision were below than 2 and 5%, respectively) and accuracy (98-102%). The limits of detection and quantification were at nanogram per milliliter (ng/mL) level. In conclusion, the simultaneous quantification of chemical markers using the proposed method is an appropriate approach for species discrimination and quality evaluation of Cecropia sp.
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