Evidence map›Paper›PMID 30765684›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2019

Resveratrol Reduces Oxidative Stress and Apoptosis in Podocytes via Sir2-Related Enzymes, Sirtuins1 (SIRT1)/Peroxisome Proliferator-Activated Receptor γ Co-Activator 1α (PGC-1α) Axis.

Tao Zhang, Yanqing Chi, Yunzhuo Ren, Chunyang Du, Yonghong Shi, Ying Li

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed, 1 pooled it
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.

  1. Pooled it
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  14. Research progress on anti-stress nutrition strategies in swine.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Tao ZhangDepartment of Nephrology, Third Hospital, Hebei Medical University, Shijiazhuang, Hebei, China (mainland).
Yanqing ChiDepartment of Nephrology, Third Hospital, Hebei Medical University, Shijiazhuang, Hebei, China (mainland).
Yunzhuo RenHebei Key Laboratory of Kidney Diseases, Shijiazhuang, Hebei, China (mainland).
Chunyang DuHebei Key Laboratory of Kidney Diseases, Shijiazhuang, Hebei, China (mainland).
Yonghong ShiHebei Key Laboratory of Kidney Diseases, Shijiazhuang, Hebei, China (mainland).
Ying LiDepartment of Nephrology, Third Hospital, Hebei Medical University, Shijiazhuang, Hebei, China (mainland).
Hebei Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND PGC-1α can be activated by deacetylation reactions catalyzed by SIRT1. Resveratrol is currently known as a potent activator of SIRT1. However, it is unknown whether the renal-protective effect of resveratrol is further related to activation of the podocyte SIRT1/PGC-1α pathway. MATERIAL AND METHODS High glucose was used to stimulate mouse podocytes. Resveratrol and PGC-1α siRNA transfection were used to perform co-intervention treatments. The protein and mRNA expression levels of SIRT1, PGC-1α, NRF1, and TFAM were detect by immunofluorescence, Western blot analysis, and qRT-PCR in the podocytes, respectively. DCHF-DA and MitoSOX™ staining were used to monitor the total ROS and mitochondrial ROS levels, respectively. The specific activities of complexes I and III were measured using Complex I and III Assay Kits. Mitochondrial membrane potential and cell apoptosis were measured using JC-1 staining and Annexin V-FITC/PI double-staining, respectively. RESULTS We found that high-glucose stimulation results in time-dependent decreases in the expression of SIRT1, PGC-1α, and its downstream genes NRF1 and mitochondrial transcription factor A (TFAM) for mouse podocytes, and increases ROS levels in cells and mitochondria. Moreover, the expression of nephrin was downregulated and the cell apoptotic rate was increased. Resveratrol treatment can improve abnormalities caused by high-glucose stimulation. In addition, it can also reduce the release of mitochondrial cytochrome C and DIABLO proteins to the cytoplasm and increase respiratory chain complex I and III activity and mitochondrial membrane potential. CONCLUSIONS Resveratrol can reduce the oxidative damage and apoptosis of podocytes induced by high-glucose stimulation via SIRT1/PGC-1α-mediated mitochondrial protection.

Indexed as

AnimalsApoptosisCell Culture TechniquesDNA-Binding ProteinsHigh Mobility Group ProteinsMembrane Potential, MitochondrialMiceMitochondriaMitochondrial ProteinsNuclear Respiratory Factor 1Oxidative StressPeroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaPodocytesProtein Processing, Post-TranslationalResveratrolRNA, Small InterferingDNA-Binding ProteinsHigh Mobility Group ProteinsMitochondrial Proteinsmitochondrial transcription factor ANrf1 protein, mouseNuclear Respiratory Factor 1Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaPpargc1a protein, mouseResveratrolRNA, Small InterferingSirt1 protein, mouseSirtuin 1Tfam protein, mouseTranscription Factors

Identifiers

PMID30765684
PMCPMC6383436
OpenAlexW2912698023

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.