ArticleMolecular systems biology2019
Temporal order and precision of complex stress responses in individual bacteria.
Article in Molecular systems biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Discovery ofmBio · 2026Article
- Dynamics and Structural Responses to Cis-Trans Isomerization in Bacterial Lipid Bilayers.ACS omega · 2026Article
- Variability in intrinsic promoter strength underlies the temporal hierarchy of the Caulobacter SOS response induction.PLoS biology · 2025Article
- Evolutionary trajectory of bacterial resistance to antibiotics and antimicrobial peptides inmSystems · 2025Article
- Added insult to injury? The response of meat-associated pathogens to proposed antimicrobial interventions.Applied microbiology and biotechnology · 2024Article
- The master regulator OxyR orchestrates bacterial oxidative stress response genes in space and time.Cell systems · 2024Article
- Stress-induced nucleoid remodeling in Deinococcus radiodurans is associated with major changes in Heat Unstable (HU) protein dynamics.Nucleic acids research · 2024Article
- Density physics-informed neural networks reveal sources of cell heterogeneity in signal transduction.Patterns (New York, N.Y.) · 2024Article
- Single-cell genomics reveals population structures fromMicrobial genomics · 2022Article
- Growth-mediated negative feedback shapes quantitative antibiotic response.Molecular systems biology · 2022Article
- The physiology and genetics of bacterial responses to antibiotic combinations.Nature reviews. Microbiology · 2022Review
- The transcription factor network of E. coli steers global responses to shifts in RNAP concentration.Nucleic acids research · 2022Article
- Growth-dependent heterogeneity in the DNA damage response in Escherichia coli.Molecular systems biology · 2022Article
- Systematic inference identifies a major source of heterogeneity in cell signaling dynamics: The rate-limiting step number.Science advances · 2022Article
- Tunable phenotypic variability through an autoregulatory alternative sigma factor circuit.Molecular systems biology · 2021Article
- Insights into the Oxidative Stress Response ofAntioxidants (Basel, Switzerland) · 2020Article
- CombiANT: Antibiotic interaction testing made easy.PLoS biology · 2020Article
- Short- and Long-Term Transcriptomic Responses of Escherichia coli to Biocides: a Systems Analysis.Applied and environmental microbiology · 2020Article
- Persistence of Intracellular Bacterial Pathogens-With a Focus on the Metabolic Perspective.Frontiers in cellular and infection microbiology · 2020Review
- Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sudden stress often triggers diverse, temporally structured gene expression responses in microbes, but it is largely unknown how variable in time such responses are and if genes respond in the same temporal order in every single cell. Here, we quantified timing variability of individual promoters responding to sublethal antibiotic stress using fluorescent reporters, microfluidics, and time-lapse microscopy. We identified lower and upper bounds that put definite constraints on timing variability, which varies strongly among promoters and conditions. Timing variability can be interpreted using results from statistical kinetics, which enable us to estimate the number of rate-limiting molecular steps underlying different responses. We found that just a few critical steps control some responses while others rely on dozens of steps. To probe connections between different stress responses, we then tracked the temporal order and response time correlations of promoter pairs in individual cells. Our results support that, when bacteria are exposed to the antibiotic nitrofurantoin, the ensuing oxidative stress and SOS responses are part of the same causal chain of molecular events. In contrast, under trimethoprim, the acid stress response and the SOS response are part of different chains of events running in parallel. Our approach reveals fundamental constraints on gene expression timing and provides new insights into the molecular events that underlie the timing of stress responses.
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