ArticlePloS one2019
Targeted fluorescence lifetime probes reveal responsive organelle viscosity and membrane fluidity.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 76 citations in OpenAlex.
- Carbohydrate-Mediated Cellular Uptake of Boronic Acid Hybrids In Cellulo and Insulin-Deficient Zebrafish: Live Imaging and Application of Multiphoton FLIM.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Polarized Phase-Sensitive Fluorescence-Image Correlation Spectroscopy.Biomolecules · 2026Article
- A Two-Photon Zn(II) Complex Photosensitizer with pH/Viscosity Dual Response for Enhanced Tumor Therapy.Molecules (Basel, Switzerland) · 2025Article
- Computational investigation of the effect of BODIPY labelling on peptide-membrane interaction.Scientific reports · 2024Article
- Age-specific and compartment-dependent changes in mitochondrial homeostasis and cytoplasmic viscosity in mouse peripheral neurons.Aging cell · 2024Article
- Fluorescent Molecular Rotors Quantify an Adjuvant-Induced Softening of Plant Wax.Chemical & biomedical imaging · 2024Article
- Fluorescence lifetime imaging microscopy of flexible and rigid dyes probes the biophysical properties of synthetic and biological membranes.Biophysical journal · 2024Article
- Review
- Molecular Rotors: Fluorescent Sensors for Microviscosity and Conformation of Biomolecules.Angewandte Chemie (International ed. in English) · 2024Review
- Optical Microscopy Using the Faraday Effect RevealsACS nano · 2024Article
- Fluorescent naphthalimide boronates as theranostics: structural investigations, confocal fluorescence and multiphoton fluorescence lifetime imaging microscopy in living cells.RSC chemical biology · 2023Article
- FluorogenicJACS Au · 2023Article
- A molecular rotor FLIM probe reveals dynamic coupling between mitochondrial inner membrane fluidity and cellular respiration.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Stress-dependent macromolecular crowding in the mitochondrial matrix.The EMBO journal · 2023Article
- Fluorescent Probes as a Tool in Diagnostic and Drug Delivery Systems.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Directly imaging emergence of phase separation in peroxidized lipid membranes.Communications chemistry · 2023Article
- How sticky? How tight? How hot? Imaging probes for fluid viscosity, membrane tension and temperature measurements at the cellular level.The international journal of biochemistry & cell biology · 2022Review
- Article
- HydroFlipper membrane tension probes: imaging membrane hydration and mechanical compression simultaneously in living cells.Chemical science · 2022Article
- Give or Take: Effects of Electron-Accepting/-Withdrawing Groups in Red-Fluorescent BODIPY Molecular Rotors.Molecules (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
The only way to visually observe cellular viscosity, which can greatly influence biological reactions and has been linked to several human diseases, is through viscosity imaging. Imaging cellular viscosity has allowed the mapping of viscosity in cells, and the next frontier is targeted viscosity imaging of organelles and their microenvironments. Here we present a fluorescent molecular rotor/FLIM framework to image both organellar viscosity and membrane fluidity, using a combination of chemical targeting and organelle extraction. For demonstration, we image matrix viscosity and membrane fluidity of mitochondria, which have been linked to human diseases, including Alzheimer's Disease and Leigh's syndrome. We find that both are highly dynamic and responsive to small environmental and physiological changes, even under non-pathological conditions. This shows that neither viscosity nor fluidity can be assumed to be fixed and underlines the need for single-cell, and now even single-organelle, imaging.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.