ArticleInternational journal of veterinary science and medicine2018
Evaluation of bull spermatozoa during and after cryopreservation: Structural and ultrastructural insights.
Article in International journal of veterinary science and medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- The Influence of L-Serine on Sperm Quality, Malondialdehyde, and Antioxidant Enzyme Activity After Cryopreservation in Bull Semen.Animals : an open access journal from MDPI · 2026Article
- Holotomography and Multivariate Analysis Reveal Donor-Specific Responses to Antioxidant Supplementation During Stallion Sperm Cryopreservation.Antioxidants (Basel, Switzerland) · 2026Article
- Probiotic Supplementation as an Alternative to Antibiotics in Bovine Sperm Cryopreservation: Effects on Motility, Oxidative Stress and Apoptosis.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Mitochondrial derived peptide humanin improved semen quality, semen freezability, antioxidant status and in-vitro fertility in crossbred bull.Scientific reports · 2026Article
- Deep learning-enabled morphology analysis of bovine sperm for label-free imaging flow cytometry.Frontiers in veterinary science · 2026Article
- Identification of fertility associated sperm sub-populations and development of quality control assays for cryopreserved semen in buffaloes (Bubalus bubalis).Veterinary research communications · 2025Article
- Sperm Membrane: Molecular Implications and Strategies for Cryopreservation in Productive Species.Animals : an open access journal from MDPI · 2025Review
- The Effect of Metallic Nanoparticles Supplementation in Semen Extender on Post-thaw Quality and Fertilizing Ability of Egyptian Buffalo (Bubalus bubalis) Spermatozoa.Biological trace element research · 2025Article
- Frozen semen quality of Pasundan bulls with different individual variations.Open veterinary journal · 2025Article
- Optimizing thyroxine levels for enhanced buffalo sperm cryopreservation and fertility: a focus on quality, viability, and antioxidant protection.Frontiers in veterinary science · 2025Article
- Ultrastructural evidence for the activation of autophagy and analysis of the protective role of autophagy in goat spermatozoa under liquid storage.Frontiers in veterinary science · 2025Article
- Effects of Ashwagandha-derived silver nanoparticles on pre- and post-thaw semen quality in bulls.Frontiers in veterinary science · 2025Article
- Protective effect of epigallocatechin-gallate in cryopreserved goat sperm on post-thawed quality.Open veterinary journal · 2025Article
- ρ-Coumaric acid-zinc oxide nanoparticles improve post-thaw quality of goat spermatozoa and developmental competence of fertilized oocytes in vitro.Scientific reports · 2024Article
- Cryoprotective Potential of Theobromine in the Improvement of the Post-Thaw Quality of Bovine Spermatozoa.Cells · 2024Article
- Cryopreservation of bovine sperm causes single-strand DNA breaks that are localized in the toroidal regions of chromatin.Journal of animal science and biotechnology · 2024Article
- The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm.In vitro cellular & developmental biology. Animal · 2024Article
- Comparative developmental competence ofVeterinary world · 2024Article
- Freezing Stallion Semen-What Do We Need to Focus on for the Future?Veterinary sciences · 2024Review
- Study on the correlation of supplementation withFrontiers in microbiology · 2024Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Semen cryopreservation is a well-established procedure used in veterinary assisted reproduction technology applications. We investigated damaging effects of cryopreservation on the structural and ultrastructural characteristics of bull sperm induced at different temperatures and steps during standard cryopreservation procedure using transmission (TEM) and scanning electron microscopy. We also examined the effect of cryopreservation on sperm DNA and chromatin integrity. Five healthy, fertile Friesian bulls were used, and the ejaculates were obtained using an artificial vagina method. The semen samples were pooled and diluted in a tris-yolk fructose (TYF) for a final concentration of 80 × 10
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