Evidence map›Paper›PMID 30760805›Full record

ArticleScientific reports2019

Label-free and Non-destruction Determination of Single- and Double-Strand DNA based on Quantum Weak Measurement.

Tian Guan, Yuxuan Yang, Qianwen Zhang, Yonghong He, Naihan Xu, Dongmei Li, Lixuan Shi, Yang Xu, Xiangnan Wang

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In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Tian GuanDepartment of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China. guantian@sz.tsinghua.edu.cn.
Yuxuan YangDepartment of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.
Qianwen ZhangKey Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China.
Yonghong HeDepartment of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China. heyh@sz.tsinghua.edu.cn.
Naihan XuKey Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China. xu.naihan@sz.tsinghua.edu.cn.
Dongmei LiShenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of optical imaging and sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.
Lixuan ShiShenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of optical imaging and sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.
Yang XuShenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of optical imaging and sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, China.
Xiangnan WangDepartment of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The process of unwinding and renaturation of DNA has been widely used in studies of nucleotide sequence organization. Compared with traditional methods for DNA unwinding and renaturation, the label-free and non-destruction detection technology is significant and desiderated. We realized an optical system based on optical rotation via weak measurement for detection of single- and double-strand state of DNA. The optical rotation, which was induced by the status change of single and double DNA strands, was exploited to modulate the preselected polarization of a weak measurement system. With this modulation, the optical rotation caused by the separation of DNA strands can be determined through the center wavelength shift of the output spectrum. By monitoring the wavelength shift in real time, the separation processes of the DNAs with different base ratio (25% and 70%) and length (4nt and 40nt), and DNAs with three terminally modified cholesterol molecules were experimentally explored in varied pH and temperature conditions. In addition, the detection limit of the DNA concentration was obtained to be 5 × 10

Indexed as

Quantum TheoryBase SequenceDNADNA, Single-StrandedHydrogen-Ion ConcentrationNucleic Acid RenaturationTemperatureDNADNA, Single-Stranded

Identifiers

PMID30760805
PMCPMC6374449

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.