ArticleScientific reports2019
Gram-scale fermentative production of ergothioneine driven by overproduction of cysteine in Escherichia coli.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 73 citations in OpenAlex.
- Engineering Escherichia coli Nissle 1917 for the highly efficient biosynthesis of food-grade ergothioneine.Applied biochemistry and biotechnology · 2026Article
- Ergothioneine: Biosynthesis, Molecular Mechanisms, Physiological Function, and Role in Disease.MedComm · 2026Review
- Efficient Synthesis of Ergothioneine in Escherichia coli Through Identification of Bacterial-Type Egt1 and Self-Assemble of Pathway Enzymes.Biotechnology and bioengineering · 2026Article
- EngineeringMicroorganisms · 2026Article
- Enhancement of ergothioneine production in Corynebacterium glutamicum by increasing osmotic pressure.Applied microbiology and biotechnology · 2025Article
- Enhanced production of ergothioneine in Aspergillus oryzae.Applied microbiology and biotechnology · 2025Article
- Biosynthesis of ergothioneine: current state, achievements, and perspectives.Applied microbiology and biotechnology · 2025Review
- Ergothioneine biosynthesis: The present state and future prospect.Synthetic and systems biotechnology · 2025Review
- AdvancingBioengineering (Basel, Switzerland) · 2024Review
- Biosynthetic ability of diverse basidiomycetous yeast strains to produce the natural antioxidant ergothioneine.AMB Express · 2024Article
- Biochemical and Structural Characterization of OvoAACS catalysis · 2023Article
- The Glutathione System: A Journey from Cyanobacteria to Higher Eukaryotes.Antioxidants (Basel, Switzerland) · 2023Review
- Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities.Synthetic and systems biotechnology · 2023Article
- Reactive Sulfur Species Produced by Cystathionine γ-lyase Function in the Establishment of Mesorhizobium loti-Lotus japonicus Symbiosis.Microbes and environments · 2023Article
- A SingleInternational journal of molecular sciences · 2022Article
- Changes in Sulfur Metabolism in Mouse Brains following Radon Inhalation.International journal of environmental research and public health · 2022Article
- Enhancement of ergothioneine production by discovering and regulating its metabolic pathway in Cordyceps militaris.Microbial cell factories · 2022Article
- A new therapy against ulcerative colitis via the intestine and brain using the Si-based agent.Scientific reports · 2022Article
- Toward more efficient ergothioneine production using the fungal ergothioneine biosynthetic pathway.Microbial cell factories · 2022Article
- Engineering ergothioneine production in Yarrowia lipolytica.FEBS letters · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ergothioneine (ERG), a unique thiol compound, is suggested to function as an antioxidant and cytoprotectant. Despite several recent attempts to produce ERG using various organisms, its yield was still very low and the costs remained high. Since the level of ERG produced depends strictly on the availability of three distinct precursor amino acids (L-cysteine (Cys), L-histidine, and L-methionine (Met)), metabolic engineering for enhancement of the flux toward ERG biosynthesis is required. Herein, we took advantage of a high-Cys production system using Escherichia coli cells, in which Cys biosynthesis and excretion were activated, and applied it to the fermentative production of ERG from glucose. The Cys overproduction in E. coli cells carrying the egtBCDE genes from Mycobacterium smegmatis was effective for ERG production. Furthermore, coexpression of the egtA gene, which encodes γ-glutamylcysteine synthetase that synthesizes the γ-glutamylcysteine used as a sulfur source of ERG biosynthesis, enhanced ERG production even though E. coli intrinsically has γ-glutamylcysteine synthetase. Additionally, disruption of the metJ gene that encodes the transcriptional repressor involved in Met metabolism was effective in further increasing the production of ERG. Finally, we succeeded in the high-level production of 1.31 g/L ERG in a fed-batch culture process using a jar fermenter.
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