ArticleNature communications2019
Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 180 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
180 citing papers in PubMed, 1 synthesis or guideline pooled it, 396 citations in OpenAlex.
- Epigenomics as Potential Tools for Enhancing Magnitude of Breeding Approaches for Developing Climate Resilient Chickpea.Frontiers in genetics · 2022Pooled it
- Abscisic Acid-Mediated Drought Tolerance inCurrent issues in molecular biology · 2026Review
- Cell-based CRISPRa screen identifies a papillae-specific receptor kinase mediating broad-spectrum fungal resistance in rice.Nature plants · 2026Article
- Transgene-free genome editing in plants.aBIOTECH · 2026Review
- Unlocking Adaptive Plasticity in Extreme Environments: Integrative Epigenomics Promises in Non-Model Plants.Plant, cell & environment · 2026Article
- Epigenome Editing for Cannabinoid Yield: Targets, Tools, and Priorities inInternational journal of molecular sciences · 2026Review
- Epigenetic orchestration of sugar signaling in plant development and stress adaptation.Horticulture research · 2026Article
- Re-evaluating the site-directed nuclease classification as a regulatory trigger for genome-edited plant products.Nature biotechnology · 2026Article
- Plant Genetic Engineering: Technological Pathways, Application Scenarios, and Future Directions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- A rapid Agrobacterium rhizogenes-mediated transient expression for assessing sgRNA efficiency in CRISPR-Act3.0 in tomato.Plant cell reports · 2026Article
- Modifying meiotic recombination by targeting chromatin regulators to crossover hotspots inScience advances · 2026Article
- Shaping the Plant Specialized Metabolites Through Modern Breeding Technique.Molecular biotechnology · 2026Review
- New insights into controlling horticultural crop quality deterioration caused by cold stress: epigenetic modification.Horticulture research · 2026Article
- Targeting dCas9-SunTag to a Susceptibility Gene Promoter Is Sufficient for CRISPR Interference.Plant direct · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Challenges and Opportunities with CRISPR-Based Genome Editing in Legume Crops.Functional & integrative genomics · 2026Review
- Research progress and applications of gene activation editing technology in crops.Frontiers in plant science · 2026Review
- Novel genome editing approaches to manipulate apical meristem activity for crop yield.Frontiers in plant science · 2026Review
- Epigenetic modifications in plant abiotic stress adaptation: towards climate-resilient and sustainable crop improvement.Frontiers in plant science · 2026Review
- The epigenetic landscape of heterosis: integrating multi-layer regulatory insights and navigating the path to causality.Frontiers in plant science · 2026Review
120 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding genomic functions requires site-specific manipulation of loci via efficient protein effector targeting systems. However, few approaches for targeted manipulation of the epigenome are available in plants. Here, we adapt the dCas9-SunTag system to engineer targeted gene activation and DNA methylation in Arabidopsis. We demonstrate that a dCas9-SunTag system utilizing the transcriptional activator VP64 drives robust and specific activation of several loci, including protein coding genes and transposable elements, in diverse chromatin contexts. In addition, we present a CRISPR-based methylation targeting system for plants, utilizing a SunTag system with the catalytic domain of the Nicotiana tabacum DRM methyltransferase, which efficiently targets DNA methylation to specific loci, including the FWA promoter, triggering a developmental phenotype, and the SUPERMAN promoter. These SunTag systems represent valuable tools for the site-specific manipulation of plant epigenomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.