ArticleGenome research2019
Dynamics of cardiomyocyte transcriptome and chromatin landscape demarcates key events of heart development.
Article in Genome research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Epigenomics and transcriptomics profiles of developing zebrafish heart cells.Scientific data · 2025Article
- PDGFRA is a conserved HAND2 effector during early cardiac development.Nature cardiovascular research · 2024Article
- PRDM16 determines specification of ventricular cardiomyocytes by suppressing alternative cell fates.Life science alliance · 2024Article
- Dual specificity and target gene selection by the MADS-domain protein FRUITFULL.Nature plants · 2023Article
- The tissue-specific chromatin accessibility landscape ofFrontiers in genetics · 2023Article
- Semaphorin3f as a cardiomyocyte derived regulator of heart chamber development.Cell communication and signaling : CCS · 2022Article
- A Novel Missense Variant in Actin Binding Domain ofFrontiers in cardiovascular medicine · 2022Article
- Multi-omics analyses of early liver injury reveals cell-type-specific transcriptional and epigenomic shift.BMC genomics · 2021Article
- A cis-regulatory-directed pipeline for the identification of genes involved in cardiac development and disease.Genome biology · 2021Article
- Matters of the heart: Cellular sex differences.Journal of molecular and cellular cardiology · 2021Review
- Conserved and species-specific chromatin remodeling and regulatory dynamics during mouse and chicken limb bud development.Nature communications · 2021Article
- Fish-Ing for Enhancers in the Heart.International journal of molecular sciences · 2021Review
- Heart Enhancers: Development and Disease Control at a Distance.Frontiers in genetics · 2021Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organogenesis involves dynamic regulation of gene transcription and complex multipathway interactions. Despite our knowledge of key factors regulating various steps of heart morphogenesis, considerable challenges in understanding its mechanism still exist because little is known about their downstream targets and interactive regulatory network. To better understand transcriptional regulatory mechanism driving heart development and the consequences of its disruption in vivo, we performed time-series analyses of the transcriptome and genome-wide chromatin accessibility in isolated cardiomyocytes (CMs) from wild-type zebrafish embryos at developmental stages corresponding to heart tube morphogenesis, looping, and maturation. We identified genetic regulatory modules driving crucial events of heart development that contained key cardiac TFs and are associated with open chromatin regions enriched for DNA sequence motifs belonging to the family of the corresponding TFs. Loss of function of cardiac TFs Gata5, Tbx5a, and Hand2 affected the cardiac regulatory networks and caused global changes in chromatin accessibility profile, indicating their role in heart development. Among regions with differential chromatin accessibility in mutants were highly conserved noncoding elements that represent putative enhancers driving heart development. The most prominent gene expression changes, which correlated with chromatin accessibility modifications within their proximal promoter regions, occurred between heart tube morphogenesis and looping, and were associated with metabolic shift and hematopoietic/cardiac fate switch during CM maturation. Our results revealed the dynamic regulatory landscape throughout heart development and identified interactive molecular networks driving key events of heart morphogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.