ArticlePlant cell reports2019
Transgenic Wucai (Brassica campestris L.) produced via Agrobacterium-mediated anther transformation in planta.
Article in Plant cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- Genome wide identification and characterization of EPFL genes and its potential association with male sterility in Brassica rapa.Scientific reports · 2025Article
- Engineering Camelina sativa Seeds as a Green Bioreactor for the Production of Affordable Human Pro-insulin that Demonstrates Anti-diabetic Efficacy in Rats.Molecular biotechnology · 2025Article
- Genome-wide identification, transcript profiling and functional analyses of PCP gene family in Wucai (Brassica campestris).Scientific reports · 2024Article
- The Highly EmbryogenicPlants (Basel, Switzerland) · 2023Article
- Amenability of anGM crops & food · 2022Article
- Article
- Genome-wide analysis of proline-rich extension-like receptor protein kinase (PERK) in Brassica rapa and its association with the pollen development.BMC genomics · 2020Article
- A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.).Journal, genetic engineering & biotechnology · 2020Article
- TheFrontiers in microbiology · 2019Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
key messageWe developed a novel Agrobacterium-mediated anther transformation for Wucai in planta, and in this procedure, the male germ line was the predominant target. Wucai (Brassica campestris L.), a variant of non-heading Chinese cabbage, is widely cultured in China and only improved by classic breeding methods. Here, a novel and efficient in planta Agrobacterium-mediated anther transformation method is developed based on the optimization of several factors that affect anther transformation. After optimization, transformation with the manual pollination application led to increased transient GUS expression in anthers (reaching 91.59%) and the transformation efficacies in planta (0.59-1.56% for four commercial cultivars). The stable integration and inheritance of the transgenes were further examined by molecular and genetic analyses. Three T
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