ArticlePediatric research2019
The mucosal-luminal interface: an ideal sample to study the mucosa-associated microbiota and the intestinal microbial biogeography.
Article in Pediatric research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- Spatial mapping of human colonic niches reveals rapid, mucus-specific microbiota disruption after bowel cleansing.Gut microbes · 2026Article
- Integrated Multi-Omics Reveals Complementary Luminal and Mucosal Host-Microbiome Interactions Associated with Disease Activity and Phenotype in Paediatric Inflammatory Bowel Disease.Microorganisms · 2026Article
- Use of Fecal Sampling for Assessing Probiotic Effects on the Gut Microbiome: A Focused Narrative Review.Cureus · 2026Review
- Complete genomes ofMicrobiology resource announcements · 2026Article
- Optimized protocol for profiling mucosa-associated microbiota from formalin-fixed paraffin-embedded gut tissues from treatment-naïve pediatric patients with Crohn's disease.Frontiers in cellular and infection microbiology · 2026Article
- The colonic mucosal virome in inflammatory bowel disease reveals Crassvirales depletion and disease-specific virome features.Gut microbes · 2025Article
- Mucosal washes are useful for sampling intestinal mucus-associated microbiota despite low biomass.Gut microbes · 2025Article
- Multi-level analysis of gut microbiome extracellular vesicles-host interaction reveals a connection to gut-brain axis signaling.Microbiology spectrum · 2025Article
- A novel method for precise endoscopic sampling of duodenal microbiota.Frontiers in cellular and infection microbiology · 2025Article
- The intestinal microbiome, but not clinical aspects of inflammatory bowel disease, is impacted by lactose malabsorption compared to lactose digestion in children.The American journal of clinical nutrition · 2024Article
- Article
- Gut-on-a-Chip Models: Current and Future Perspectives for Host-Microbial Interactions Research.Biomedicines · 2023Review
- HIV-Positive Patients on Antiretroviral Therapy Have an Altered Mucosal Intestinal but Not Oral Microbiome.Microbiology spectrum · 2023Article
- Diversity of the microbiota communities found in the various regions of the intestinal tract in healthy individuals and inflammatory bowel diseases.Frontiers in immunology · 2023Review
- Relationship between mucosa-associated gut microbiota and human diseases.Biochemical Society transactions · 2022Review
- Untangling the oral-gut axis in the pathogenesis of intestinal inflammation.International immunology · 2022Review
- Article
- Gut and airway microbiota and their role in COVID-19 infection and pathogenesis: a scoping review.Infection · 2022Article
- Gut-Skin Axis: Unravelling the Connection between the Gut Microbiome and Psoriasis.Biomedicines · 2022Review
- Assessing the Impact of Diet on the Mucosa-Adhered Microbiome in Piglets Using Comparative Analysis of Rectal Swabs and Colon Content.Frontiers in microbiology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
backgroundAlterations in gastrointestinal microbial communities have been linked to human disease. Most studies use fecal samples as a proxy for the intestinal microbiota; however, the fecal microbiome is not fully representative of the mucosa-associated microbiota at the site of disease. While mucosal biopsies can be used instead, they often contain a high proportion of host DNA that can confound 16S ribosomal RNA (rRNA) gene sequencing studies.
methodsTo overcome these limitations, we sampled the mucosal-luminal interface (MLI) to study the mucosa-associated microbiota. We also employed a simple bioinformatics workflow to remove contaminants from 16S rRNA gene profiling results.
resultsOur results indicate that the microbial differences between individuals are greater than those between different microenvironments within the same individual. Moreover, biopsy samples frequently contained contaminants that could significantly impact biopsy profiling results.
conclusionsOur findings highlight the utility of collecting MLI aspirates to complement biopsies and stools for characterizing human microbial communities.
Indexed as
Identifiers
30758325What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.