ArticlePlant reproduction2019
Step-by-step protocols for rice gamete isolation.
Article in Plant reproduction, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Premeiotic chromatin states orchestrate gene expression during male gametogenesis in rice.Genome biology · 2026Article
- The roles of a novel CDKB/KRP/FB3 cell cycle core complex in rice gametes and initiation of embryogenesis.Plant reproduction · 2023Article
- Paternal DNA methylation is remodeled to maternal levels in rice zygote.Nature communications · 2023Article
- Fifty years of sperm cell isolations: from structural to omic studies.Journal of experimental botany · 2023Article
- Resetting of the 24-nt siRNA landscape in rice zygotes.Genome research · 2022Article
- Plant sexual reproduction: perhaps the current plant two-sex model should be replaced with three- and four-sex models?Plant reproduction · 2021Article
- Isolation of the sperm and egg cells in wild rice (Oryza officinalis) as a mechanism for crop improvement.Plant reproduction · 2020Article
- Genome-wide redistribution of 24-nt siRNAs in rice gametes.Genome research · 2020Article
- Special issue: cellular omics methods in plant reproduction research.Plant reproduction · 2019Article
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Authors and funding
4 authors.
Funding
Abstract
key messageA detailed, step-by-step protocol for isolation of rice gametes for transcriptional profiling, with a general workflow that includes controls for RNA contamination from surrounding cells and tissues is presented. Characterization of the transcriptome and other -omics studies of flowering plant gametes are challenging as a consequence of the small sizes and relative inaccessibility of these cells. Collecting such poorly represented cells is also complicated by potential contamination from surrounding sporophytic, adjacent gametophytic tissues and difficulties in extracting high-quality intact cells. Here we present detailed, step-by-step procedures for collecting intact, unfixed rice (Oryza sativa) egg cells and sperm cells without enzymatic treatments. In addition, we also present a general workflow for assessing sample purity by RT-PCR, using primers specific for marker genes preferentially expressed in surrounding cells and tissues. These protocols should facilitate future studies of genome-scale characterization of gametes in this important model crop.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.