Evidence map›Paper›PMID 30756174›Full record

ArticleJournal of bone and mineral metabolism2019

Modulation of bone turnover by Cissus quadrangularis after ovariectomy in rats.

Juan M Guerra, Martha A Hanes, Cordelia Rasa, Nagarajan Loganathan, Wendy Innis-Whitehouse, Ednia Gutierrez, Saraswathy Nair, Jameela Banu

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of bone and mineral metabolism, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Juan M GuerraDepartment of Health and Biomedical Sciences, University of Texas Rio Grande Valley, 1201, W University Dr., Edinburg, TX, 78539, USA.
Martha A HanesDepartment of Lab Animal Resources, University of Texas Health at San Antonio, 7703 Floyd Curl Dr, San Antonio, TX 78229, USA.
Cordelia RasaDepartment of Lab Animal Resources, University of Texas Rio Grande Valley, 1201, W University Dr, Edinburg, TX 78539, USA.
Nagarajan LoganathanPoorveegam Research Trust, Pondicherry, India.
Wendy Innis-WhitehouseSchool of Medicine, University of Texas Rio Grande Valley, 1201 W University Dr, Edinburg, TX, 78539, USA.
Ednia GutierrezDepartment of Health and Biomedical Sciences, University of Texas Rio Grande Valley, 1201, W University Dr., Edinburg, TX, 78539, USA.
Saraswathy NairDepartment of Health and Biomedical Sciences, University of Texas Rio Grande Valley, 1201, W University Dr., Edinburg, TX, 78539, USA.
Jameela BanuDepartment of Health and Biomedical Sciences, University of Texas Rio Grande Valley, 1201, W University Dr., Edinburg, TX, 78539, USA. Jameela.banu@utrgv.edu.
The University of Texas Rio Grande Valley · USThe University of Texas Health Science Center at San Antonio · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In women, age-related bone loss is associated with increased risk of bone fracture. Existing therapies are associated with severe side effects; thus, there is a need to find alternative medicines with less or optimal side effects. Cissus quadrangularis (CQ), an Ayurvedic medicine used to enhance fracture healing, was tested for its bone protective properties and studied to discern the mechanism by which it is beneficial to bone. Female Sprague Dawley rats were either sham operated or ovariectomized and were fed CQ for 3 months. Several biochemical markers, cytokines and hormones were assayed. Femur, tibia and lumbar vertebrae were subjected to pQCT and µCT densitometry. MC3T3 cells were cultured, treated with CQ and used to analyze miRNA content and subjected to qPCR for gene expression analysis related to bone metabolism. CQO rats showed protected bone mass and microarchitecture of trabecular bone in the distal femoral metaphysis and the proximal tibial metaphysis. The lumbar vertebrae, however, showed no significant changes. Serum protein expression levels of P1NP increased and Trap5b and CTX levels decreased with in vivo CQ treatment. Some influence on the anti- and pro-inflammatory markers was also observed. Significantly high level of estradiol in the CQO rats was observed. In vitro expression of a few genes related to bone metabolism showed that osteocalcin increased significantly. The other genes-collagen I expression, SPP1, BMP2, DCAT1-decreased significantly. Certain miRNA that regulate bone turnover using the BMP pathway and Wnt signaling pathways were upregulated by CQ. qPCR after acute treatment with CQ showed significantly increased levels of osteocalcin and decreased levels of Wnt/β catenin antagonist DCAT1. Overall, CQ protected the microarchitecture of the long bones from ovariectomy-induced bone loss. This may be because of decreased inflammation and modulation through the BMP and Wnt signaling pathways. We conclude that CQ is a potential therapeutic agent to treat postmenopausal osteoporosis with no side effects.

Indexed as

Bone RemodelingOvariectomyAnimalsBiomarkersBody WeightCell LineCissusCytokinesFeeding BehaviorFemaleHormonesHumansLipidsLiverLumbar VertebraeMiceBiomarkersCytokinesHormonesLipidsMicroRNAsPlant ExtractsBone biochemical markersCissus quadrangularismiRNA and qPCRPostmenopausal bone lossStatic histomorphometry and bone strength

Identifiers

PMID30756174
OpenAlexW2912920433

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.