ArticleScientific reports2019
Development of a new High Resolution Melting (HRM) assay for identification and differentiation of Mycobacterium tuberculosis complex samples.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Haplotype and Presence-Absence Variation Analysis Using High-Resolution Melting (HRM) Analysis for Genotyping Genes of Interest.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Culture-independent detection ofFrontiers in veterinary science · 2025Article
- A Novel Approach Based on Real-Time PCR with High-Resolution Melting Analysis for the Simultaneous Identification ofFoods (Basel, Switzerland) · 2024Article
- Genomic comparison betweenFrontiers in veterinary science · 2024Article
- A novel multiplex qPCR‑HRM assay for the simultaneous detection of four abortive zoonotic agents in cattle, sheep, and goats.Scientific reports · 2023Article
- Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples.Scientific reports · 2022Article
- Optimization and development of high-resolution melting curve analysis (HRMA) assay for detection of New Delhi metallo-β-lactamase (NDM) producingAIMS microbiology · 2022Article
- Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis.Scientific reports · 2021Article
- Efficacy of 16S rRNA variable regions high-resolution melt analysis for bacterial pathogens identification in periprosthetic joint infections.BMC microbiology · 2021Article
- Single-nucleotide polymorphism-based epidemiological analysis of KoreanJournal of veterinary science · 2021Article
- Accurate Identification of Closely RelatedFrontiers in cellular and infection microbiology · 2021Article
- Efficient differentiation ofJournal of medical microbiology · 2020Article
- Article
- Prevalence and Molecular Typing of Colistin-ResistantInfection and drug resistance · 2020Article
- Development of high-resolution melting curve analysis in rapid detection ofTropical medicine and health · 2020Article
- Three-reaction high-resolution melting assay for rapid differentiation of Mycobacterium tuberculosis complex members.MicrobiologyOpen · 2019Article
- Development and application of affordable SNP typing approaches to genotype Mycobacterium tuberculosis complex strains in low and high burden countries.Scientific reports · 2019Article
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3 authors.
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Abstract
The rapid identification and differentiation of members of the Mycobacterium tuberculosis complex (MTBC) is essential to assess the potential zoonotic risk. Different available molecular methods are time consuming since they depend on cultivation of mycobacteria. High Resolution Melting (HRM) is a low cost, rapid and easy to perform single-tube method not limited to cultured samples. In this study, a HRM assay specifically targeting gyrB was developed to simultaneously identify and differentiate Mycobacterium (M.) tuberculosis, M. microti and M. bovis/M. caprae. To evaluate the performance of this assay, 38 MTBC isolates and 25 directly extracted clinical specimens were analysed. HRM results of all 38 (100%) examined isolates correlated with the results obtained with the commercially available GenoType MTBC test (Hain Lifescience). From the 25 clinical specimens tested, species identification by HRM showed concordant results with the previously used identification methods in 23 samples (92%). The assay demonstrated a good analytical sensitivity, specificity and reproducibility and can be used directly on clinical specimens.
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