Evidence map›Paper›PMID 30755613›Full record

ArticleNature communications2019

A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface ATP.

Mark A Lobas, Rongkun Tao, Jun Nagai, Mira T Kronschläger, Philip M Borden, Jonathan S Marvin, Loren L Looger, Baljit S Khakh

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 178 papers.

0numbers the graph read from it
0cells of the map it votes in
178citing papers in PubMed
23.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

178 citing papers in PubMed, 309 citations in OpenAlex.

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118 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 3 countries.

Mark A LobasDepartment of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095-1751, USA.
Rongkun TaoDepartment of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095-1751, USA.
Jun NagaiDepartment of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095-1751, USA.
Mira T KronschlägerDepartment of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095-1751, USA.ORCID http://orcid.org/0000-0002-3216-5243
Philip M BordenJanelia Research Campus, 19700 Helix Drive, Ashburn, VA, 20147, USA.ORCID http://orcid.org/0000-0003-1653-7067
Jonathan S MarvinJanelia Research Campus, 19700 Helix Drive, Ashburn, VA, 20147, USA.
Loren L LoogerJanelia Research Campus, 19700 Helix Drive, Ashburn, VA, 20147, USA. loogerl@janelia.hhmi.org.ORCID http://orcid.org/0000-0002-7531-1757
Baljit S KhakhDepartment of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095-1751, USA. bkhakh@mednet.ucla.edu.
University of California, Los Angeles · USJanelia Research Campus · US

Funding

Astrocyte branchlet dysfunction as an early step in brain disordersDP1MH104069 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KHAKH, BALJIT · 2013 to 2017
$3.9M
A genetically-encoded sensor for imaging extracellular ATP onto astrocytes and other cellsR21NS100525 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KHAKH, BALJIT · 2016 to 2017
$424k
NIMH NIH HHS DP1 MH104069NINDS NIH HHS R21 NS100525
6 · The paper itself

Abstract

Adenosine 5' triphosphate (ATP) is a universal intracellular energy source and an evolutionarily ancient, ubiquitous extracellular signal in diverse species. Here, we report the generation and characterization of single-wavelength genetically encoded fluorescent sensors (iATPSnFRs) for imaging extracellular and cytosolic ATP from insertion of circularly permuted superfolder GFP into the epsilon subunit of F

Indexed as

Adenosine TriphosphateATPase Inhibitory ProteinBacillusBacterial ProteinsCell MembraneCytosolFluorescence Resonance Energy TransferGene ExpressionHEK293 CellsHumansHydrogen-Ion ConcentrationImage Processing, Computer-AssistedKineticsLuminescent ProteinsMicroscopy, FluorescenceModels, MolecularAdenosine TriphosphateATPase Inhibitory ProteinBacterial ProteinsLuminescent ProteinsProteinsRed Fluorescent Protein

Identifiers

PMID30755613
PMCPMC6372613
OpenAlexW2952574460

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.