ArticleG3 (Bethesda, Md.)2019
Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast.
Article in G3 (Bethesda, Md.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- A CRISPR/Cas9-induced blunt-end telomere system in S. pombe reveals RNase H2-dependent RNA primer removal at the terminal Okazaki fragment of lagging telomeres.Nucleic acids research · 2026Article
- Enhancing CRISPR Homology Directed Repair in IAL-PiD2 Insect Cells via Reagent Delivery Optimization and Cell Synchronization.Biochemical engineering journal · 2026Article
- CRISPR-Cas9 Genome Editing in Auxotrophic and Non-auxotrophic Fission Yeast Strains.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Creating Meiotic Recombination-Regulating DNA Sites byBiomolecules · 2024Article
- The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing.Synthetic and systems biotechnology · 2023Review
- Review
- Optimization of the CRISPR/Cas9 system usingmicroPublication biology · 2023Article
- Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors.The CRISPR journal · 2022Article
- Easy efficient HDR-based targeted knock-in inBioengineered · 2022Article
- Protein acetylation regulates xylose metabolism during adaptation of Saccharomyces cerevisiae.Biotechnology for biofuels · 2021Article
- Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2021Article
- New insights into the construction of wild-type Saba pig-derived3 Biotech · 2021Article
- Advances in Accurate Microbial Genome-Editing CRISPR Technologies.Journal of microbiology and biotechnology · 2021Review
- Targeted mutagenesis in human iPSCs using CRISPR genome-editing tools.Methods (San Diego, Calif.) · 2021Article
- Implementation of dCas9-mediated CRISPRi in the fission yeast Schizosaccharomyces pombe.G3 (Bethesda, Md.) · 2021Article
- CRISPR/Cas-based precision genome editing via microhomology-mediated end joining.Plant biotechnology journal · 2021Review
- Genome editing systems across yeast species.Current opinion in biotechnology · 2020Review
- Nutrient-dependent control of RNA polymerase II elongation rate regulates specific gene expression programs by alternative polyadenylation.Genes & development · 2020Article
- CRISPR-Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference.Nucleic acids research · 2020Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR/Cas9 system enables the editing of genomes of numerous organisms through the induction of the double-strand breaks (DSB) at specific chromosomal targets. We improved the CRISPR/Cas9 system to ease the direct introduction of a point mutation or a tagging sequence into the chromosome by combining it with the noncanonical homology-directed DNA repair (HDR) based genome editing in fission yeast. We constructed convenient cloning vectors, which possessed a guide RNA (gRNA) expression module, or the humanized
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.