Evidence map›Paper›PMID 30754715›Full record

ArticleCells2019

Purification of Functional Human TRP Channels Recombinantly Produced in Yeast.

Liying Zhang, Kaituo Wang, Dan Arne Klaerke, Kirstine Calloe, Lillian Lowrey, Per Amstrup Pedersen, Pontus Gourdon, Kamil Gotfryd

Open access · goldAbstract read
In one paragraph

Article in Cells, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Production of an Active, Human Membrane Protein inInternational journal of molecular sciences · 2022
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Liying ZhangDepartment of Biomedical Sciences, University of Copenhagen, Nørre Allé 14, DK-2200 Copenhagen N, Denmark. liying.zhang@sund.ku.dk.
Kaituo WangDepartment of Biomedical Sciences, University of Copenhagen, Nørre Allé 14, DK-2200 Copenhagen N, Denmark. kaituo@sund.ku.dk.
Dan Arne KlaerkeDepartment of Veterinary and Animal Sciences, University of Copenhagen, Dyrlægevej 100, DK-1870 Frederiksberg C, Denmark. dk@sund.ku.dk.
Kirstine CalloeDepartment of Veterinary and Animal Sciences, University of Copenhagen, Dyrlægevej 100, DK-1870 Frederiksberg C, Denmark. kirstinec@sund.ku.dk.
Lillian LowreyDepartment of Veterinary and Animal Sciences, University of Copenhagen, Dyrlægevej 100, DK-1870 Frederiksberg C, Denmark. lillianclowrey@gmail.com.
Per Amstrup PedersenDepartment of Biology, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen OE, Denmark. papedersen@bio.ku.dk.
Pontus GourdonDepartment of Biomedical Sciences, University of Copenhagen, Nørre Allé 14, DK-2200 Copenhagen N, Denmark. pontus@sund.ku.dk.
Kamil GotfrydDepartment of Biomedical Sciences, University of Copenhagen, Nørre Allé 14, DK-2200 Copenhagen N, Denmark. kamil@sund.ku.dk.
University of Copenhagen · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

(1) Background: Human transient receptor potential (TRP) channels constitute a large family of ion-conducting membrane proteins that allow the sensation of environmental cues. As the dysfunction of TRP channels contributes to the pathogenesis of many widespread diseases, including cardiac disorders, these proteins also represent important pharmacological targets. TRP channels are typically produced using expensive and laborious mammalian or insect cell-based systems. (2) Methods: We demonstrate an alternative platform exploiting the yeast

Indexed as

AnimalsDetergentsHumansPhylogenyRecombinant ProteinsSaccharomyces cerevisiaeSolubilityTransient Receptor Potential ChannelsDetergentsRecombinant ProteinsTransient Receptor Potential Channelsion channelsoverproductionproduction platformprotein purificationSaccharomyces cerevisiaesensorstransient receptor potential (TRP) channelsyeast

Identifiers

PMID30754715
PMCPMC6406451
OpenAlexW2911426246

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.