ArticleBiology of reproduction2019
A high-throughput method for unbiased quantitation and categorization of nuclear morphology†.
Article in Biology of reproduction, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 60 citations in OpenAlex.
- Testicular toll with viral intrusion: Molecular insights into the impact of chronic HIV infection on male fertility.Clinical and translational medicine · 2026Article
- Loss-of-Function SSFA2 Variants Disrupt Fertility Through Impaired Acrosome Formation and Sperm Function.Andrology · 2026Article
- Testis-specific lncRNA Teshl regulates acrosome biogenesis to maintain sperm structure and function.Cell & bioscience · 2026Article
- Epididymal sperm maturation in mouse species with differing levels of sperm competition†.Biology of reproduction · 2026Article
- Beyond swimming: emerging parameters for predicting the fertility of mouse spermatozoa.Lab animal · 2025Review
- Effect of Probiotics on Sperm Quality in the Adult Mouse.Probiotics and antimicrobial proteins · 2025Article
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- Phenotypic continuum and poor intracytoplasmic sperm injection intracytoplasmic sperm injection prognosis in patients harboring HENMT1 variants.Andrology · 2025Article
- Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis.Nucleic acids research · 2025Article
- Systematic identification of Y-chromosome gene functions in mouse spermatogenesis.Science (New York, N.Y.) · 2025Article
- Constitutive Androstane Receptor Regulates Germ Cell Homeostasis, Sperm Quality, and Male Fertility via Akt-Foxo1 Pathway.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- AnBiomicrofluidics · 2024Article
- Genetic mutation ofLife science alliance · 2024Article
- Article
- Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa.International journal of biological sciences · 2023Article
- The contribution of sex chromosome conflict to disrupted spermatogenesis in hybrid house mice.Genetics · 2022Article
- PFN4 is required for manchette development and acrosome biogenesis during mouse spermiogenesis.Development (Cambridge, England) · 2022Article
- Loss of Prm1 leads to defective chromatin protamination, impaired PRM2 processing, reduced sperm motility and subfertility in male mice.Development (Cambridge, England) · 2022Article
- Loss of the cleaved-protamine 2 domain leads to incomplete histone-to-protamine exchange and infertility in mice.PLoS genetics · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
The physical arrangement of chromatin in the nucleus is cell type and species-specific, a fact particularly evident in sperm, in which most of the cytoplasm has been lost. Analysis of the characteristic falciform ("hook shaped") sperm in mice is important in studies of sperm development, hybrid sterility, infertility, and toxicology. However, quantification of sperm shape differences typically relies on subjective manual assessment, rendering comparisons within and between samples difficult. We have developed an analysis program for morphometric analysis of asymmetric nuclei and characterized the sperm of mice from a range of inbred, outbred, and wild-derived mouse strains. We find that laboratory strains have elevated sperm shape variability both within and between samples in comparison to wild-derived inbred strains, and that sperm shape in F1 offspring from a cross between CBA and C57Bl6J strains is subtly affected by the direction of the cross. We further show that hierarchical clustering can discriminate distinct sperm shapes with greater efficiency and reproducibility than even experienced manual assessors, and is useful both to distinguish between samples and also to identify different morphological classes within a single sample. Our approach allows for the analysis of nuclear shape with unprecedented precision and scale and will be widely applicable to different species and different areas of biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.