ArticlePloS one2019
Salmonella-vectored vaccine delivering three Clostridium perfringens antigens protects poultry against necrotic enteritis.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Combatting Salmonella: a focus on antimicrobial resistance and the need for effective vaccination.BMC infectious diseases · 2025Pooled it
- Protection against necrotic enteritis in broiler chickens by co-administration of Enterococcus faecalis EF32 and recombinant Lactobacillus plantarum strain co-expressing FimA, FimB, NetB and Fba.Poultry science · 2026Article
- An engineered Escherichia coli-ClyA-NetB outer membrane vesicle delivery system modulates jejunal microbiota in broiler chickens with necrotic enteritis.Poultry science · 2026Article
- Sortase-mediated surface display of a chimeric immunogen on Bacillus subtilis: a chicken necrotic enteritis vaccine candidate.Microbial cell factories · 2025Article
- In Silico Design, Optimization, and Evaluation of a Multi-Epitope Vaccine Targeting theMicroorganisms · 2025Article
- Oral and parenteral vaccination of broiler chickens with Recombinant NetB antigen from Clostridium perfringens confers significant protection against necrotic enteritis.BMC veterinary research · 2025Article
- Genetic engineering ofEcoSal Plus · 2024Review
- Recombinant Live-AttenuatedVaccines · 2024Review
- Necrotic enteritis and antibiotic-free production of broiler chickens: Challenges in testing and using alternative products.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Review
- A comprehensive review of experimental models and induction protocols for avian necrotic enteritis over the past 2 decades.Frontiers in veterinary science · 2024Review
- Toxinotyping ofIranian journal of veterinary research · 2024Article
- A novel bacterium-like particles platform displaying antigens by new anchoring proteins induces efficacious immune responses.Frontiers in microbiology · 2024Article
- Immunization of broiler chickens with five newly identified surface-exposed proteins unique to Clostridium perfringens causing necrotic enteritis.Scientific reports · 2023Article
- Evaluation the efficacy of oral immunization of broiler chickens with a recombinant Lactobacillus casei vaccine vector expressing the Carboxy-terminal fragment of α-toxin from Clostridium perfringens.BMC veterinary research · 2023Article
- Lactobacillus casei displaying Clostridium perfringens NetB antigen protects chickens against necrotic enteritis.Applied microbiology and biotechnology · 2022Article
- Immunization with Pooled Antigens forVaccines · 2022Article
- Vaccines UsingMicroorganisms · 2022Article
- Recombinant Limosilactobacillus (Lactobacillus) delivering nanobodies against Clostridium perfringens NetB and alpha toxin confers potential protection from necrotic enteritis.MicrobiologyOpen · 2022Article
- Efficacy of Multivalent, Cochleate-Based Vaccine againstVaccines · 2022Article
- Bioengineered Probiotics: Synthetic Biology Can Provide Live Cell Therapeutics for the Treatment of Foodborne Diseases.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Necrotic enteritis is an economically important poultry disease caused by the bacterium Clostridium perfringens. There are currently no necrotic enteritis vaccines commercially available for use in broiler birds, the most important target population. Salmonella-vectored vaccines represent a convenient and effective option for controlling this disease. We used a single attenuated Salmonella vaccine strain, engineered to lyse within the host, to deliver up to three C. perfringens antigens. Two of the antigens were toxoids, based on C. perfringens α-toxin and NetB toxin. The third antigen was fructose-1,6-bisphosphate aldolase (Fba), a metabolic enzyme with an unknown role in virulence. Oral immunization with a single Salmonella vaccine strain producing either Fba, α-toxoid and NetB toxoid, or all three antigens, was immunogenic, inducing serum, cellular and mucosal responses against Salmonella and the vectored C. perfringens antigens. All three vaccine strains were partially protective against virulent C. perfringens challenge. The strains delivering Fba only or all three antigens provided the best protection. We also demonstrate that both toxins and Fba are present on the C. perfringens cell surface. The presence of Fba on the cell surface suggests that Fba may function as an adhesin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.