ArticlePLoS neglected tropical diseases2019
Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Dengue vaccine acceptability in Peru: A mixed-methods study in two dengue-endemic Peruvian cities.PLoS neglected tropical diseases · 2026Article
- Entomological Surveillance and Vector Competence: A Crucial Front in Dengue Prevention.Current topics in microbiology and immunology · 2026Review
- Rhesus macaques model human Mayaro virus disease and transmit to Aedes aegypti mosquitoes.PLoS neglected tropical diseases · 2025Article
- Trade-offs shaping transmission of sylvatic dengue and Zika viruses in monkey hosts.Nature communications · 2024Article
- Vector competence of Aedes albopictus field populations from Reunion Island exposed to local epidemic dengue viruses.PloS one · 2024Article
- Direct mosquito feedings on dengue-2 virus-infected people reveal dynamics of human infectiousness.PLoS neglected tropical diseases · 2023Article
- Evaluation of "Caserotek" a low cost and effective artificial blood-feeding device for mosquitoes.PLoS neglected tropical diseases · 2023Article
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- Potential for community based surveillance of febrile diseases: Feasibility of self-administered rapid diagnostic tests in Iquitos, Peru and Phnom Penh, Cambodia.PLoS neglected tropical diseases · 2021Article
- Considerations for Human Blood-Feeding and Arthropod Exposure in Vector Biology Research: An Essential Tool for Investigations and Disease Control.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2020Review
- Vector Competence: What Has Zika Virus Taught Us?Viruses · 2019Review
- Acceptability of Aedes aegypti blood feeding on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru.PLoS neglected tropical diseases · 2019Article
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Authors and funding
20 authors at 6 institutions in 3 countries.
Funding
Abstract
backgroundTransmission of dengue virus (DENV) from humans to mosquitoes represents a critical component of dengue epidemiology. Examinations of this process have generally been hampered by a lack of methods that adequately represent natural acquisition of DENV by mosquitoes from humans. In this study, we assessed artificial and natural blood feeding methods based on rates of DENV infection and dissemination within mosquitoes for use in a field-based epidemiological cohort study in Iquitos, Peru. METHODOLOGY/PRINCIPAL
findingsOur study was implemented, stepwise, between 2011 and 2015. Participants who were 5 years and older with 5 or fewer days of fever were enrolled from ongoing clinic- and neighborhood-based studies on dengue in Iquitos. Wild type, laboratory-reared Aedes aegypti were fed directly on febrile individuals or on blood collected from participants that was either untreated or treated with EDTA. Mosquitoes were tested after approximately 14 days of extrinsic incubation for DENV infection and dissemination. A total of 58 participants, with viremias ranging from 1.3 × 10(2) to 2.9 × 10(6) focus-forming units per mL of serum, participated in one or more feeding methods. DENV infection and dissemination rates were not significantly different following direct and indirect-EDTA feeding; however, they were significantly lower for mosquitoes that fed indirectly on blood with no additive. Relative to direct feeding, infection rates showed greater variation following indirect-EDTA than indirect-no additive feeding. Dissemination rates were similar across all feeding methods. No differences were detected in DENV infection or dissemination rates in mosquitoes fed directly on participants with different dengue illness severity. CONCLUSIONS/SIGNIFICANCE: Our study demonstrates the feasibility of using direct and indirect feeding methods for field-based studies on vector competence. Direct mosquito feeding is preferable in terms of logistical ease, biosecurity, and reliability.
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