ArticleIn vitro cellular & developmental biology. Animal2019
Genetic algorithm as an optimization tool for the development of sponge cell culture media.
Article in In vitro cellular & developmental biology. Animal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Coating materials enhance urochordate primary cell culture adherence.In vitro cellular & developmental biology. Animal · 2026Article
- An in vitro cellular model for measuring the impact of thermal stress on Florida reef sponges.In vitro cellular & developmental biology. Animal · 2026Article
- Article
- Genetically tractable embryonic cell lines from sea urchins Lytechinus variegatus and Strongylocentrotus purpuratus.Communications biology · 2025Article
- Advancing marine invertebrate cell line research: four key knowledge gaps.In vitro cellular & developmental biology. Animal · 2025Review
- Review
- Candidatus Nemesobacterales is a sponge-specific clade of the candidate phylum Desulfobacterota adapted to a symbiotic lifestyle.The ISME journal · 2023Article
- Taurine as a key intermediate for host-symbiont interaction in the tropical sponge Ianthella basta.The ISME journal · 2023Article
- Improved Media Formulations for Primary Cell Cultures Derived from a Colonial Urochordate.Cells · 2023Article
- First continuous marine sponge cell line established.Scientific reports · 2023Article
- A review of algorithmic approaches for cell culture media optimization.Frontiers in bioengineering and biotechnology · 2023Review
- Comparative Genomics Provides Insight into the Function of Broad-Host Range Sponge Symbionts.mBio · 2021Article
- 3-D Culture of Marine Sponge Cells for Production of Bioactive Compounds.Marine drugs · 2021Article
- Cultivation of fractionated cells from a bioactive-alkaloid-bearing marine sponge Axinella sp.In vitro cellular & developmental biology. Animal · 2021Article
- Stem Cells and Innate Immunity in Aquatic Invertebrates: Bridging Two Seemingly Disparate Disciplines for New Discoveries in Biology.Frontiers in immunology · 2021Review
- Breakthrough in Marine Invertebrate Cell Culture: Sponge Cells Divide Rapidly in Improved Nutrient Medium.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Sponges are rich sources of novel natural products. Production in cell cultures may be an option for supply of these compounds but there are currently no sponge cell lines. Because there is a lack of understanding about the precise conditions and nutritional requirements that are necessary to sustain sponge cells in vitro, there has yet to be a defined, sponge-specific nutrient medium. This study utilized a genetic algorithm approach to optimize the amino acid composition of a commercially available basal cell culture medium in order to increase the metabolic activity of cells of the marine sponge Dysidea etheria. Four generations of the algorithm were carried out in vitro in wet lab conditions and an optimal medium combination was selected for further evaluation. When compared to the basal medium control, there was a twofold increase in metabolic activity. The genetic algorithm approach can be used to optimize other components of culture media to efficiently optimize chosen parameters without the need for detailed knowledge on all possible interactions.
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