ArticleJournal of materials science. Materials in medicine2019
Micropatterning of endothelial cells to create a capillary-like network with defined architecture by laser-assisted bioprinting.
Article in Journal of materials science. Materials in medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 85 citations in OpenAlex.
- Vascularized bone organoids: current advances and a biomimetic platform for osteonecrosis of the femoral head.Bone research · 2026Review
- From Printability to Biofunctionality: 3D-Printed Hydrogel Scaffolds for Multi-Tissue Engineering.Gels (Basel, Switzerland) · 2026Review
- Coupling intraoperative bioprinting and surgical micropuncture for synergistic scaffold vascularization.Biomaterials · 2026Article
- A Quantitative Printability Framework for Programmable Assembly of Pre-Vascular Patterns via Laser-Induced Forward Transfer.Advanced healthcare materials · 2026Article
- 3D-Bioprinted Oil-Based Hydrogels: A Sustainable Approach for Bone and Dental Regeneration.International journal of molecular sciences · 2025Review
- Integrating microfluidic and bioprinting technologies: advanced strategies for tissue vascularization.Lab on a chip · 2025Review
- Leveraging printability and biocompatibility in materials for printing implantable vessel scaffolds.Materials today. Bio · 2024Review
- Integrating bioprinting, cell therapies and drug delivery towards in vivo regeneration of cartilage, bone and osteochondral tissue.Drug delivery and translational research · 2024Review
- Technology for the formation of engineered microvascular network models and their biomedical applications.Nano convergence · 2024Review
- Advancements of 3D bioprinting in regenerative medicine: Exploring cell sources for organ fabrication.Heliyon · 2024Review
- Advances in 3D bioprinting for regenerative medicine applications.Regenerative biomaterials · 2024Review
- Keeping It Organized: Multicompartment Constructs to Mimic Tissue Heterogeneity.Advanced healthcare materials · 2023Review
- Review
- Nanocomposite Bioprinting for Tissue Engineering Applications.Gels (Basel, Switzerland) · 2023Review
- Bioprinting Technologies and Bioinks for Vascular Model Establishment.International journal of molecular sciences · 2023Review
- Advances in 3D skin bioprinting for wound healing and disease modeling.Regenerative biomaterials · 2023Review
- Research progress of vascularization strategies of tissue-engineered bone.Frontiers in bioengineering and biotechnology · 2023Review
- Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing.Bioactive materials · 2022Review
- Engineering Spatiotemporal Control in Vascularized Tissues.Bioengineering (Basel, Switzerland) · 2022Review
- Hydrogel: A Potential Material for Bone Tissue Engineering Repairing the Segmental Mandibular Defect.Polymers · 2022Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Development of a microvasculature into tissue-engineered bone substitutes represents a current challenge. Seeding of endothelial cells in an appropriate environment can give rise to a capillary-like network to enhance prevascularization of bone substitutes. Advances in biofabrication techniques, such as bioprinting, could allow to precisely define a pattern of endothelial cells onto a biomaterial suitable for in vivo applications. The aim of this study was to produce a microvascular network following a defined pattern and preserve it while preparing the surface to print another layer of endothelial cells. We first optimise the bioink cell concentration and laser printing parameters and then develop a method to allow endothelial cells to survive between two collagen layers. Laser-assisted bioprinting (LAB) was used to pattern lines of tdTomato-labeled endothelial cells cocultured with mesenchymal stem cells seeded onto a collagen hydrogel. Formation of capillary-like structures was dependent on a sufficient local density of endothelial cells. Overlay of the pattern with collagen I hydrogel containing vascular endothelial growth factor (VEGF) allowed capillary-like structures formation and preservation of the printed pattern over time. Results indicate that laser-assisted bioprinting is a valuable technique to pre-organize endothelial cells into high cell density pattern in order to create a vascular network with defined architecture in tissue-engineered constructs based on collagen hydrogel.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.