ArticleScience advances2019
Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures.
Article in Science advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The trial behind it
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Who cites it
23 citing papers in PubMed.
- DPEP2 suppresses hyperinflammation via metabolic reprogramming of macrophages in sepsis.Nature communications · 2026Article
- Immune signatures of megakaryocytes in persistent inflammation-immunosuppression and catabolism syndrome.Acta biochimica et biophysica Sinica · 2025Article
- Exploring Core Genes Associated with Sepsis and Systemic Inflammatory Response Syndrome Using Single-Cell Sequencing Technology.Journal of inflammation research · 2025Article
- The Role of Dectin-1-Akt-RNF146 Pathway in β-Glucan Induced Immune Trained State of Monocyte in Sepsis.Journal of inflammation research · 2025Article
- Microliter Whole Blood Neutrophil Assay Preserving Physiological Lifespan and Functional Heterogeneity.Small methods · 2024Article
- Heat Inactivation of Nipah Virus for Downstream Single-Cell RNA Sequencing Does Not Interfere with Sample Quality.Pathogens (Basel, Switzerland) · 2024Article
- Establishment of an Inactivation Method for Ebola Virus and SARS-CoV-2 Suitable for Downstream Sequencing of Low Cell Numbers.Pathogens (Basel, Switzerland) · 2023Article
- Recent advances in microfluidic-based cancer immunotherapy-on-a-chip strategies.Biomicrofluidics · 2023Review
- Titration of 124 antibodies using CITE-Seq on human PBMCs.Scientific reports · 2022Article
- Single-Cell Sequencing in Rheumatic Diseases: New Insights from the Perspective of the Cell Type.Aging and disease · 2022Article
- Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cells.BMC biology · 2022Article
- ReDisX, a machine learning approach, rationalizes rheumatoid arthritis and coronary artery disease patients uniquely upon identifying subpopulation differentiation markers from their genomic data.Frontiers in medicine · 2022Article
- Abnormal Changes of Monocyte Subsets in Patients With Sjögren's Syndrome.Frontiers in immunology · 2022Article
- Peripheral Blood-Based Biomarkers for Immune Checkpoint Inhibitors.International journal of molecular sciences · 2021Review
- Plasma from patients with bacterial sepsis or severe COVID-19 induces suppressive myeloid cell production from hematopoietic progenitors in vitro.Science translational medicine · 2021Article
- Cell type prioritization in single-cell data.Nature biotechnology · 2021Article
- Integrative genomic analysis reveals mechanisms of immune evasion in P. falciparum malaria.Nature communications · 2020Article
- Scalable Signature-Based Molecular Diagnostics Through On-chip Biomarker Profiling Coupled with Machine Learning.Annals of biomedical engineering · 2020Review
- Genome-wide whole blood transcriptome profiling in a large European cohort of systemic sclerosis patients.Annals of the rheumatic diseases · 2020Article
- Engineering Microphysiological Immune System Responses on Chips.Trends in biotechnology · 2020Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Specialized immune cell subsets are involved in autoimmune disease, cancer immunity, and infectious disease through a diverse range of functions mediated by overlapping pathways and signals. However, subset-specific responses may not be detectable in analyses of whole blood samples, and no efficient approach for profiling cell subsets at high throughput from small samples is available. We present a low-input microfluidic system for sorting immune cells into subsets and profiling their gene expression. We validate the system's technical performance against standard subset isolation and library construction protocols and demonstrate the importance of subset-specific profiling through in vitro stimulation experiments. We show the ability of this integrated platform to identify subset-specific disease signatures by profiling four immune cell subsets in blood from patients with systemic lupus erythematosus (SLE) and matched control subjects. The platform has the potential to make multiplexed subset-specific analysis routine in many research laboratories and clinical settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.