ArticleNeurophotonics2019
Fluorescence lifetime imaging nanoscopy for measuring Förster resonance energy transfer in cellular nanodomains.
Article in Neurophotonics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Theoretical Design of All-Optical Universal Logic via Multistage FRET-STED Energy Cascades in Fluorescent Protein Nanoclusters.ACS omega · 2026Article
- Enhancing STED microscopy via fluorescence lifetime unmixing and filtering in two-species SPLIT-STED.Methods in microscopy · 2026Article
- Fluorescence nanoscopy at the sub-10 nm scale.Biophysical reviews · 2021Review
- FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.eLife · 2021Review
- A Guide to Fluorescence Lifetime Microscopy and Förster's Resonance Energy Transfer in Neuroscience.Current protocols in neuroscience · 2020Review
- Synthetic non-classical luminescence generation by enhanced silica nanophotonics based on nano-bio-FRET.RSC advances · 2020Article
- Imaging the Neuroimmune Dynamics Across Space and Time.Frontiers in neuroscience · 2020Review
- The in vivo mechanics of the magnetotactic backbone as revealed by correlative FLIM-FRET and STED microscopy.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microscopy methods used to measure Förster resonance energy transfer (FRET) between fluorescently labeled proteins can provide information on protein interactions in cells. However, these methods are diffraction-limited, thus do not enable the resolution of the nanodomains in which such interactions occur in cells. To overcome this limitation, we assess FRET with an imaging system combining fluorescence lifetime imaging microscopy with stimulated emission depletion, termed fluorescence lifetime imaging nanoscopy (FLIN). The resulting FRET-FLIN approach utilizes immunolabeling of proteins in fixed cultured neurons. We demonstrate the capacity to discriminate nanoclusters of synaptic proteins exhibiting variable degrees of interactions with labeled binding partners inside dendritic spines of hippocampal neurons. This method enables the investigation of FRET within nanodomains of cells, approaching the scale of molecular signaling.
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Registered trials
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