ArticleJournal of tissue engineering
Development of a tissue-engineered skin substitute on a base of human amniotic membrane.
Article in Journal of tissue engineering. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Sterilization of Human Amniotic Membrane Using an Ozone Hydrodynamic System.Annals of biomedical engineering · 2024Article
- Application of Amniotic Membrane in Skin Regeneration.Pharmaceutics · 2023Review
- Comparison of human amniotic membrane decellularisation approaches for hESC-derived RPE cells culture.BMJ open ophthalmology · 2022Article
- Bioengineering Vascular Networks to Study Angiogenesis and Vascularization of Physiologically Relevant Tissue ModelsACS biomaterials science & engineering · 2020Article
- Evaluation of Radiosterilized Glyercerolated Amniotic Membranes as a Substrate for Cultured Human Epithelial Cells.Organogenesis · 2020Article
- Evaluation of Tissue-engineered Skin on Base of Human Amniotic Membrane for Wound Healing.Plastic and reconstructive surgery. Global open · 2019Article
- Regulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering.International journal of nanomedicine · 2019Article
- Recent update on craniofacial tissue engineering.Journal of tissue engineeringReview
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Allogenic graft material and tissue engineering have recently shown promising results for the improvement of both esthetic and functional outcomes in the treatment of large skin defects. We chose human amniotic membrane as a cellular scaffold in order to develop a skin substitute for later in vivo uses. Various methods of de-epithelialization of the human amniotic membrane were evaluated by histological analysis including hematoxylin-eosin and laminin staining, optic coherence tomography, and scanning electron microscopy with 0.25/0.02% trypsin/ethylenediaminetetraacetic acid treatment and mechanical cell removal showing an almost complete loss of the epithelium and a mainly intact basement membrane. Novel examination of human amniotic membrane by optic coherence tomography was feasible, but difficulties were experienced in handling and interpretation of the tissue as no comparable data exist. Subsequently, we developed an air-liquid interface cell culture to cultivate keratinocytes and fibroblasts on the de-epithelialized human amniotic membrane. We achieved a mostly keratinized surface on the epidermal side with a confluent fibroblast network on the chorion side.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.