ArticleChemical science2019
A lithium-ion-active aerolysin nanopore for effectively trapping long single-stranded DNA.
Article in Chemical science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- High sensitivity detection of Hepatitis B virus RNA based on 3D-DNA nanomachine and protein nanopore sensing.Molecular biomedicine · 2025Article
- Nanopore approaches for single-molecule temporal omics: promises and challenges.Nature methods · 2025Review
- Deep Learning-Assisted Single-Molecule Detection of Protein Post-translational Modifications with a Biological Nanopore.ACS nano · 2024Article
- Biological nanopores for single-molecule sensing.iScience · 2022Review
- An engineered third electrostatic constriction of aerolysin to manipulate heterogeneously charged peptide transport.Chemical science · 2022Article
- Development of the DNA-based biosensors for high performance in detection of molecular biomarkers: More rapid, sensitive, and universal.Biosensors & bioelectronics · 2022Review
- Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants.RSC advances · 2021Review
- In situ food-borne pathogen sensors in a nanoconfined space by surface enhanced Raman scattering.Mikrochimica acta · 2021Review
- A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides.Plant communications · 2021Article
- Designed alpha-helical barrels for charge-selective peptide translocation.Chemical science · 2020Article
- Dynamic interaction of fluoroquinolones with magnesium ions monitored using bacterial outer membrane nanopores.Chemical science · 2020Article
- Insight into the effects of electrochemical factors on host-guest interaction induced signature events in a biological nanopore.Nanotechnology and Precision Engineering · 2020Article
- Direct Quantification of Damaged Nucleotides in Oligonucleotides Using an Aerolysin Single Molecule Interface.ACS central science · 2020Article
- Enzymatic reaction-based nanopore detection of zinc ions.The Analyst · 2019Article
- Locus-patterned sequence oriented enrichment for multi-dimensional gene analysis.Chemical science · 2019Article
- A Nanopore Phosphorylation Sensor for Single Oligonucleotides and Peptides.Research (Washington, D.C.) · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wild-type aerolysin (AeL) nanopores allow direct single nucleotide discrimination of very short oligonucleotides (≤10 nt) without labelling, which shows great potential for DNA sensing. To achieve real applications, one major obstacle of AeL is its poor capture ability of long single-stranded DNA (ssDNA, >10 nt). Here, we have proposed a novel and robust strategy for the electrostatic focusing of long ssDNA into a lithium-chloride (LiCl)-active AeL. By using this method, for the first time we have demonstrated AeL detection of ssDNA longer than 100 nt. Due to screening more negative charges, LiCl improves AeL capture ability of long ssDNA (
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