Evidence map›Paper›PMID 30745377›Full record

ArticleJournal of bacteriology2019

A Xylose-Inducible Expression System and a CRISPR Interference Plasmid for Targeted Knockdown of Gene Expression in Clostridioides difficile.

Ute Müh, Anthony G Pannullo, David S Weiss, Craig D Ellermeier

Open access · bronzeAbstract read
In one paragraph

Article in Journal of bacteriology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.

0numbers the graph read from it
0cells of the map it votes in
57citing papers in PubMed
4.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

57 citing papers in PubMed, 72 citations in OpenAlex.

  1. Article
  2. Defining the order of assembly of theJournal of bacteriology · 2026
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  5. CenIR, an essential BlaIR-family regulatory system inbioRxiv : the preprint server for biology · 2026
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  6. Pharmaceutics · 2026
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  7. A set of genetic tools for use inMicrobiology (Reading, England) · 2026
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  8. Article
  9. Defining the order of assembly of thebioRxiv : the preprint server for biology · 2026
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  10. Article
  11. Article
  12. Article
  13. Article
  14. Analysis of essential genes inJournal of bacteriology · 2025
    Article
  15. Article
  16. Review
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  18. Article
  19. Analysis of Essential Genes inbioRxiv : the preprint server for biology · 2025
    Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Ute MühDepartment of Microbiology and Immunology, University of Iowa, Iowa City, Iowa, USA.
Anthony G PannulloDepartment of Microbiology and Immunology, University of Iowa, Iowa City, Iowa, USA.
David S WeissDepartment of Microbiology and Immunology, University of Iowa, Iowa City, Iowa, USA david-weiss@uiowa.edu craig-ellermeier@uiowa.edu.
Craig D EllermeierDepartment of Microbiology and Immunology, University of Iowa, Iowa City, Iowa, USA david-weiss@uiowa.edu craig-ellermeier@uiowa.edu.ORCID 0000-0002-8953-5081
University of Iowa · US

Funding

TRAINING IN MECHANISMS OF PARASITISMT32AI007511 · NIAID · UNIVERSITY OF IOWA · PI BUTLER, NOAH SULLIVAN, WEBER, MARY · 1996 to 2025
$8.1M
Identification of daptomycin resistance mechanisms in Clostridioides difficileR01AI087834 · NIAID · UNIVERSITY OF IOWA · PI ELLERMEIER, CRAIG D · 2011 to 2025
$5.6M
Regulation of toxin gene expression in Clostridium difficileR21AI121576 · NIAID · UNIVERSITY OF IOWA · PI ELLERMEIER, CRAIG D · 2016 to 2017
$417k
NIAID NIH HHS R01 AI087834NIAID NIH HHS R21 AI121576NIAID NIH HHS T32 AI007511
6 · The paper itself

Abstract

Here we introduce plasmids for xylose-regulated expression and repression of genes in

Indexed as

Clustered Regularly Interspaced Short Palindromic RepeatsGene Knockdown TechniquesBacterial ProteinsClostridioides difficileGene ExpressionGene Expression Regulation, BacterialGenes, ReporterGenetic VectorsPlasmidsXyloseBacterial ProteinsXyloseClostridioides difficileClostridium difficileCRISPR interferencegene expressiongenetics

Identifiers

PMID30745377
PMCPMC6597395
OpenAlexW2900748336

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.