ArticleCell chemical biology2019
Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags.
Article in Cell chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed.
- Chemical Biology 2025: Highlights From the Ch/Bi145 Course at Caltech.Chembiochem : a European journal of chemical biology · 2026Review
- Technologies to measure and modulate protein subcellular localization.Nature reviews. Molecular cell biology · 2026Review
- Phenoxazines with a PhototransferableJournal of the American Chemical Society · 2026Article
- aPKC and F-actin dynamics promote Hippo pathway polarity in asymmetrically dividing neuroblasts.Biology open · 2026Article
- Chemical biology approaches to study and target circadian clocks and their components.FEBS letters · 2026Review
- Utilizing the predictable binding kinetics of DNA-PAINT to denoise super-resolution images.Nature communications · 2026Article
- Dual-Color Expansion Microscopy of Membrane Proteins Using Bioorthogonal Labeling.Nano letters · 2026Article
- A general one-step protocol to generate impermeable fluorescent HaloTag substrates for in situ live cell application and super-resolution imaging.Nature communications · 2026Article
- Synthetic fluorophores for live-cell fluorescence microscopy and biosensing.Nature chemical biology · 2025Review
- Nanoscale spatiotemporal cluster analysis of expressed and endogenous proteins.Nature protocols · 2025Review
- Single-cell synaptome mapping of endogenous protein subpopulations in mammalian brain.Nature communications · 2025Article
- SNAP-tag2 for faster and brighter protein labeling.Nature chemical biology · 2025Article
- Rapid and Uniform NHS-Ester-Based Membrane Protein Labeling of Live Mammalian Cells.Bio-protocol · 2025Article
- Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.Chemical & biomedical imaging · 2025Review
- Developing HaloTag and SNAP-Tag Chemical Inducers of Dimerization to Probe Receptor Oligomerization and Downstream Signaling.Angewandte Chemie (International ed. in English) · 2025Article
- Super-resolution microscopy for structural biology.Nature methods · 2025Review
- Thioether editing generally increases the photostability of rhodamine dyes on self-labeling tags.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Resolution in super-resolution microscopy - facts, artifacts, technological advancements and biological applications.Journal of cell science · 2025Article
- Unveiling cellular communications through rapid pan-membrane-protein labeling.Nature communications · 2025Article
24 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors.
Funding
Abstract
Super-resolution microscopy requires that subcellular structures are labeled with bright and photostable fluorophores, especially for live-cell imaging. Organic fluorophores may help here as they can yield more photons-by orders of magnitude-than fluorescent proteins. To achieve molecular specificity with organic fluorophores in live cells, self-labeling proteins are often used, with HaloTags and SNAP-tags being the most common. However, how these two different tagging systems compare with each other is unclear, especially for stimulated emission depletion (STED) microscopy, which is limited to a small repertoire of fluorophores in living cells. Herein, we compare the two labeling approaches in confocal and STED imaging using various proteins and two model systems. Strikingly, we find that the fluorescent signal can be up to 9-fold higher with HaloTags than with SNAP-tags when using far-red rhodamine derivatives. This result demonstrates that the labeling strategy matters and can greatly influence the duration of super-resolution imaging.
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