ArticleBMC biotechnology2019
Production of high titer of citric acid from inulin.
Article in BMC biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 38 citations in OpenAlex.
- New Yarrowia lipolytica chassis strains for industrial enzyme production.Microbial cell factories · 2025Article
- Effects and function of citric acid on fermentation quality and microbial community in sugarcane tops silage with high and low water-soluble carbohydrate content.BMC plant biology · 2025Article
- Advancements in metabolic engineering: unlocking the potential of key organic acids for sustainable industrial applications.Frontiers in bioengineering and biotechnology · 2025Review
- Application of NewInternational journal of molecular sciences · 2024Article
- Citric Acid: Properties, Microbial Production, and Applications in Industries.Molecules (Basel, Switzerland) · 2023Review
- Growth Response of Non-Conventional Yeasts on Sugar-Rich Media: Part 2: Citric Acid Production and Circular-Oriented Valorization of Glucose-Enriched Olive Mill Wastewaters Using NovelMicroorganisms · 2023Article
- Metabolic engineering ofSynthetic and systems biotechnology · 2023Article
- Modelling of the Citric Acid Production from Crude Glycerol by Wild-TypeLife (Basel, Switzerland) · 2022Article
- Review
- Yeast Protein as an Easily Accessible Food Source.Metabolites · 2022Review
- Optimization of Yarrowia lipolytica-based consolidated biocatalyst through synthetic biology approach: transcription units and signal peptides shuffling.Applied microbiology and biotechnology · 2020Article
- EngineeringMicroorganisms · 2020Article
- Sugar Alcohols and Organic Acids Synthesis inMicroorganisms · 2020Review
- Organic Wastes as Feedstocks for Non-Conventional Yeast-Based Bioprocesses.Microorganisms · 2019Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCitric acid is considered as the most economically feasible product of microbiological production, therefore studies on cheap and renewable raw materials for its production are highly desirable. In this study citric acid was synthesized by genetically engineered strains of Yarrowia lipolytica from widely available, renewable polysaccharide - inulin. Hydrolysis of inulin by the Y. lipolytica strains was established by expressing the inulinase gene (INU1 gene; GenBank: X57202.1) with its native secretion signal sequence was amplified from genomic DNA from Kluyveromyces marxianus CBS6432. To ensure the maximum citric acid titer, the optimal cultivation strategy-repeated-batch culture was applied.
resultsThe strain Y. lipolytica AWG7 INU 8 secreted more than 200 g dm
conclusionsThe citric acid titer obtained in the proposed process is the highest value reported in the literature for Yarrowia yeast. The obtained results suggest that citric acid production from inulin by engineered Y. lipolytica may be a very promising technology for industrial citric acid production.
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