Evidence map›Paper›PMID 30744049›Full record

ArticleViruses2019

Divergent Evolution of E1A CR3 in Human Adenovirus Species D.

Gurdeep Singh, Ashrafali M Ismail, Jeong Yoon Lee, Mirja Ramke, Ji Sun Lee, David W Dyer, Donald Seto, Jaya Rajaiya, James Chodosh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Gurdeep SinghDepartment of Ophthalmology, Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA. gur.singh@mail.utoronto.ca.ORCID 0000-0003-2519-0456
Ashrafali M IsmailDepartment of Ophthalmology, Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA. Mohamed_Ismail@meei.harvard.edu.ORCID 0000-0001-8694-7235
Jeong Yoon LeeDepartment of Ophthalmology, Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA. jeongyoon.lee@jbnu.ac.kr.
Mirja RamkeDepartment of Ophthalmology, Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA. mirja.ramke@gmx.de.
Ji Sun LeeDepartment of Ophthalmology, Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA. ljs02122013@gmail.com.
David W DyerDepartment of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. david-dyer@ouhsc.edu.
Donald SetoBioinformatics and Computational Biology Program, School of Systems Biology, George Mason University, Manassas, VA 22030, USA. dseto@gmu.edu.
Jaya RajaiyaDepartment of Ophthalmology, Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA. jaya_rajaiya@meei.harvard.edu.
James ChodoshDepartment of Ophthalmology, Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA. james_chodosh@meei.harvard.edu.ORCID 0000-0002-7463-1599
Massachusetts Eye and Ear Infirmary · USGeorge Mason University · USUniversity of Oklahoma Health Sciences Center · US

Funding

Proteomics CoreP20GM103447 · NIGMS · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI Mark L Lang · 2012 to 2026
$60.2M
P30 Core Grant for Vision ResearchP30EY014104 · NEI · MASSACHUSETTS EYE AND EAR INFIRMARY · PI Janey L Wiggs · 2002 to 2026
$15.1M
Immunopathogenesis of Adenovirus KeratitisR01EY013124 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI James Chodosh, Jaya Rajaiya · 2001 to 2026
$10.8M
Novel Mechanisms in Adenoviral Ocular PathogenesisR01EY021558 · NEI · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI James Chodosh, Jaya Rajaiya · 2011 to 2026
$7.1M
NEI NIH HHS P30 EY014104NEI NIH HHS R01 EY013124NEI NIH HHS R01 EY021558NIGMS NIH HHS P20 GM103447
6 · The paper itself

Abstract

Adenovirus E1A is the first viral protein expressed during infection. E1A controls critical aspects of downstream viral gene expression and cell cycle deregulation, and its function is thought to be highly conserved among adenoviruses. Various bioinformatics analyses of E1A from 38 human adenoviruses of species D (HAdV-D), including likelihood clade model partitioning, provided highly significant evidence of divergence of HAdV-Ds into two distinct groups for the conserved region 3 (CR3), present only in the E1A 13S isoform. This variance within E1A 13S of HAdV-Ds was not found in any other human adenovirus (HAdV) species. By protein sequence and structural analysis, the zinc finger motif of E1A CR3, previously shown as critical for transcriptional activation, showed the greatest differences. Subsequent codon usage bias analysis revealed substantial divergence in E1A 13S between the two groups of HAdV-Ds, suggesting that these two sub-groups of HAdV-D evolved under different cellular conditions. Hence, HAdV-D E1A embodies a previously unappreciated evolutionary divergence among HAdVs.

Indexed as

Evolution, MolecularZinc FingersAdenovirus E1A ProteinsAdenoviruses, HumanComputational BiologyConserved SequenceGene Expression Regulation, ViralHumansProtein IsoformsSequence Analysis, DNATranscriptional ActivationAdenovirus E1A ProteinsProtein IsoformsadenovirusE1Aviral evolution

Identifiers

PMID30744049
PMCPMC6409611
OpenAlexW2914396866

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.