Evidence map›Paper›PMID 30742896›Full record

ArticleJournal of structural biology2019

Enhancement of tomogram interpretability using the locked self-rotation function.

Andrei Fokine, Baldeep Khare, Yingyuan Sun, Michael G Rossmann

Abstract read
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In one paragraph

Article in Journal of structural biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Andrei FokineDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, United States. Electronic address: afokine@purdue.edu.
Baldeep KhareDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, United States.
Yingyuan SunDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, United States.
Michael G RossmannDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, United States. Electronic address: mr@purdue.edu.

Funding

Structure and Function of FlavivirusesR01AI076331 · NIAID · PURDUE UNIVERSITY · PI KUHN, RICHARD J. · 2008 to 2017
$6.8M
Structural studies of bacteriophage T4 and their potential medical applicationsR01AI081726 · NIAID · PURDUE UNIVERSITY · PI KUHN, RICHARD J., RAO, VENIGALLA B. · 2010 to 2019
$6.2M
Structural studies of bacteriophage T4 and their potential medical applicationsR56AI081726 · NIAID · PURDUE UNIVERSITY · PI ROSSMANN, MICHAEL G · 2009 to 2009
$600k
NIAID NIH HHS R01 AI076331NIAID NIH HHS R01 AI081726NIAID NIH HHS R56 AI081726
6 · The paper itself

Abstract

The interpretation of cryo-electron tomograms of macromolecular complexes can be difficult because of the large amount of noise and because of the missing wedge effect. Here it is shown how the presence of rotational symmetry in a sample can be utilized to enhance the quality of a tomographic analysis. The orientation of symmetry axes in a sub-tomogram can be determined using a locked self-rotation function. Given this knowledge, the sub-tomogram density can then be averaged to improve its interpretability. Sub-tomograms of the icosahedral bacteriophage phiX174 are used to demonstrate the procedure.

Indexed as

Bacteriophage phi X 174CapsidCryoelectron MicroscopyElectron Microscope TomographyImage Processing, Computer-AssistedRotationCryo-electron tomographyLocked self-rotation functionMacromolecular complexesPoint group symmetryVirus structure

Identifiers

PMID30742896
PMCPMC6420866

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.