ArticleMolecular microbiology2019
The master quorum-sensing regulators LuxR/HapR directly interact with the alpha subunit of RNA polymerase to drive transcription activation in Vibrio harveyi and Vibrio cholerae.
Article in Molecular microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- GplR1, an unusual TetR-like transcription factor inmSystems · 2025Article
- Quorum sensing regulates virulence factors in the coral pathogenApplied and environmental microbiology · 2025Article
- Quorum sensing in Vibrio controls carbon metabolism to optimize growth in changing environmental conditions.PLoS biology · 2024Article
- Pseudomonas aeruginosa regulator PvrA binds simultaneously to multiple pseudo-palindromic sites for efficient transcription activation.Science China. Life sciences · 2024Article
- Identification of critical amino acids in the DNA binding domain of LuxO: Lessons from a constitutive active LuxO.PloS one · 2024Article
- Engineering ofInternational journal of molecular sciences · 2022Article
- Amino acid divergence in the ligand-binding pocket of Vibrio LuxR/HapR proteins determines the efficacy of thiophenesulfonamide inhibitors.Molecular microbiology · 2021Article
- Overcoming stochastic variations in culture variables to quantify and compare growth curve data.BioEssays : news and reviews in molecular, cellular and developmental biology · 2021Article
- Article
- Biosynthesis pathways and strategies for improving 3-hydroxypropionic acid production in bacteria.World journal of microbiology & biotechnology · 2021Review
- The DNA binding domain of the Vibrio vulnificus SmcR transcription factor is flexible and binds diverse DNA sequences.Nucleic acids research · 2021Article
- Binding site profiles and N-terminal minor groove interactions of the master quorum-sensing regulator LuxR enable flexible control of gene activation and repression.Nucleic acids research · 2021Article
- Purification of the Vibrio Quorum-Sensing Transcription Factors LuxR, HapR, and SmcR.Methods in molecular biology (Clifton, N.J.) · 2021Article
- ChiS is a noncanonical DNA-binding hybrid sensor kinase that directly regulates the chitin utilization program inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Global H-NS counter-silencing by LuxR activates quorum sensing gene expression.Nucleic acids research · 2020Article
- Quorum-Sensing Regulator OpaR Directly Represses Seven Protease Genes inFrontiers in microbiology · 2020Article
- HilD induces expression of a novel Salmonella Typhimurium invasion factor, YobH, through a regulatory cascade involving SprB.Scientific reports · 2019Article
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2 authors at 1 institution in 1 country.
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Abstract
In Vibrio species, quorum sensing controls gene expression for numerous group behaviors, including bioluminescence production, biofilm formation, virulence factor secretion systems, and competence. The LuxR/HapR master quorum-sensing regulators activate expression of hundreds of genes in response to changes in population densities. The mechanism of transcription activation by these TetR-type transcription factors is unknown, though LuxR DNA binding sites that lie in close proximity to the -35 region of the promoter are required for activation at some promoters. Here, we show that Vibrio harveyi LuxR directly interacts with RNA polymerase to activate transcription of the luxCDABE bioluminescence genes. LuxR interacts with RNA polymerase in vitro and in vivo and specifically interacts with both the N- and C-terminal domains of the RNA polymerase α-subunit. Amino acid substitutions in the RNAP interaction domain on LuxR decrease interactions between LuxR and the α-subunit and result in defects in transcription activation of quorum-sensing genes in vivo. The RNAP-LuxR interaction domain is conserved in Vibrio cholerae HapR and is required for activation of the HapR-regulated gene hapA. Our findings support a model in which LuxR/HapR bind proximally to RNA polymerase to drive transcription initiation at a subset of quorum-sensing genes in Vibrio species.
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