ArticleScientific reports2019
Particle Diffusometry: An Optical Detection Method for Vibrio cholerae Presence in Environmental Water Samples.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- A smartphone-based particle diffusometry platform for sub-attomolar detection of Vibrio cholerae in environmental water.Biosensors & bioelectronics · 2020Trial
- Optical biosensing using particle diffusometry on thermoplastic microfluidic chips bonded using direct and indirect chip bonding methods.Biomedical microdevices · 2026Article
- Real-Time Visualization of HIV-1 RNA Detection Using Loop-Mediated Isothermal Amplification-Enabled Particle Diffusometry.ACS sensors · 2024Article
- Biomolecular Interaction Analysis Quantification with a Low-Volume Microfluidic Chip and Particle Diffusometry.Analytical chemistry · 2024Article
- Temporally and spatially resolved micro-rheometry of a transient viscous polymer formation.Measurement science & technology · 2023Article
- Quantifying Brownian motion in the presence of simple shear flow with particle diffusometry.Experiments in fluids · 2023Article
- Review of Rapid Diagnostic Methods forThe East African health research journal · 2023Article
- LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.Biosensors · 2022Review
- Translating diagnostics and drug delivery technologies to low-resource settings.Science translational medicine · 2022Review
- Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA.Biosensors & bioelectronics · 2022Article
- Ultrafast and Sensitive Screening of Pathogens by Functionalized Janus Microbeads-Enabled Rotational Diffusometry in Combination with Isothermal Amplification.Small science · 2022Article
- PD-LAMP smartphone detection of SARS-CoV-2 on chip.Analytica chimica acta · 2022Article
- Towards the use of a smartphone imaging-based tool for point-of-care detection of asymptomatic low-density malaria parasitaemia.Malaria journal · 2021Article
- Pocket MUSE: an affordable, versatile and high-performance fluorescence microscope using a smartphone.Communications biology · 2021Article
- Article
- Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples.Lab on a chip · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a need for a rapid, robust, and sensitive biosensor to identify low concentrations of pathogens in their native sample matrix without enrichment or purification. Nucleic acid-based detection methods are widely accepted as the gold standard in diagnostics, but robust detection of low concentrations of pathogens remains challenging. Amplified nucleic acids produce more viscous solutions, which can be measured by combining these products with fluorescent particles and measuring the change in the particle diffusion coefficient using a technique known as particle diffusometry. Here, we utilize Vibrio cholerae (V. cholerae) as a proof-of-concept for our detection system due to its inherently low concentration in environmental water samples. We demonstrate that particle diffusometry can be used to detect down to 1 V. cholerae cell in molecular-grade water in 20 minutes and 10 V. cholerae cells in pond water in just 35 minutes in 25 µL reaction volumes. The detection limit in pond water is environmentally relevant and does not require any enrichment or sample preparation steps. Particle diffusometry is 10-fold more sensitive than current gold standard fluorescence detection of nucleic acid amplification. Therefore, this novel measurement technique is a promising approach to detect low levels of pathogens in their native environments.
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